Mohammad Lamiaa A, Atef Dina M, Abul-Saoud Amany Mustafa
Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.
Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.
Hum Immunol. 2015 Oct;76(10):724-8. doi: 10.1016/j.humimm.2015.09.027. Epub 2015 Sep 30.
Monocyte chemoattractant protein-1 (MCP-1) is a member of CC chemokine that plays an important role in the recruitment of monocytes/macrophages into renal tubulointerstitium. A biallelic A/G polymorphism at position ∼2518 in the MCP-1 gene was found to regulate MCP-1 expression. MCP-1 and its A/G gene polymorphism have been implicated in the pathogenesis of some renal diseases. The aim of the study was to investigate the role of the MCP-1 gene polymorphism as early predictors of the development of glomerulonephropathy in SLE patients. We also aimed to measure the serum and urinary levels of MCP-1 in patients with SLE, to find out its relation to clinical disease activity.
140 SLE patients (100 with nephritis and 40 without nephritis) and 80 controls were included in this study. MCP-1 gene polymorphism was analyzed by polymerase chain reaction. Serum and urine MCP-1 level were measured using high-sensitivity enzyme-linked immunosorbent assay.
The A/A genotype was more common in controls than in SLE patients, whereas both the A/G (P<0.000) and G/G (P<0.000) genotypes were more frequent in SLE patients. Carriers of G allele of the MCP-1 ∼2518 polymorphism had more than 7 fold increased risk to develop glomerulo-nephropathy in patients with SLE. High MCP-1 circulating levels production from patients with A/G and G/G genotypes was significantly higher than in A/A genotype. In addition there were significant differences in the mean levels of serum MCP-1 (P<0.001) and urinary MCP-1 (P<0.001) between patients and controls.
The present study provides a new evidence that the presence of MCP-1 A (-2518) G gene polymorphism and high circulating MCP-1 levels can play an important role in the development of SLE and nephropathy in Egyptians.
单核细胞趋化蛋白-1（MCP-1）是CC趋化因子家族的一员，在单核细胞/巨噬细胞募集至肾小管间质过程中起重要作用。已发现MCP-1基因约2518位存在双等位基因A/G多态性可调节MCP-1表达。MCP-1及其A/G基因多态性与某些肾脏疾病的发病机制有关。本研究旨在探讨MCP-1基因多态性作为系统性红斑狼疮（SLE）患者肾小球肾炎发生早期预测指标的作用。我们还旨在检测SLE患者血清和尿液中MCP-1水平，以明确其与临床疾病活动度的关系。
本研究纳入140例SLE患者（100例有肾炎，40例无肾炎）和80例对照。采用聚合酶链反应分析MCP-1基因多态性。使用高灵敏度酶联免疫吸附测定法检测血清和尿液MCP-1水平。
A/A基因型在对照组中比在SLE患者中更常见，而A/G（P<0.000）和G/G（P<0.000）基因型在SLE患者中更常见。MCP-1约2518多态性的G等位基因携带者发生SLE患者肾小球肾炎的风险增加7倍以上。A/G和G/G基因型患者的MCP-1循环水平显著高于A/A基因型。此外，患者与对照组之间血清MCP-1平均水平（P<0.001）和尿液MCP-1平均水平（P<0.001）存在显著差异。
本研究提供了新的证据，表明MCP-1 A（-2518）G基因多态性的存在和高循环MCP-1水平在埃及人SLE和肾病的发生中起重要作用。
