白细胞介素-37抑制体外培养的SMMC-7721细胞的增殖、侵袭和迁移

[IL-37 inhibits the proliferation, invasion and migration of SMMC-7721 cells in vitro].

作者信息

Zhang Guoqing, Tang Chengyoug, Tan Jingyu, Liu Rui, Zhou Mi, Wu Zhongjun

机构信息

Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Oct;31(10):1301-5.

PMID:26429527

Abstract

OBJECTIVE

To analyze the impact of interleukin-37 (IL-37) on proliferation, migration and invasion of SMMC-7721 hepatocellular carcinoma cells and the related molecular mechanism.

METHODS

SMMC-7721 cells cultured in vitro were divided into the (50, 100, 200, 500) ng/mL recombinant human IL-37 (rhIL-37) treated groups and control group. Cell proliferation ability was detected by CCK-8 assay. Wound healing assay was used to observe cell migration ability. Transwell(TM) assay was performed to test the cell invasion ability. The levels of signal transducers and activators of transcription 3 (STAT3), phospho-STAT3 (p-STAT3) and matrix metalloproteinase-2 (MMP-2) were determined using Western blotting.

RESULTS

CCK-8 assay showed that rhIL-37 inhibited the proliferation of SMMC-7721 cells. Wound healing assay revealed that rhIL-37 inhibited the migration of SMMC-7721 cells. Transwell(TM) indicated that rhIL-37 inhibited the invasion of SMMC-7721 cells. Western blotting demonstrated that rhIL-37 down-regulated the expressions of p-STAT3 and MMP-2.

CONCLUSION

IL-37 can inhibit the proliferation, migration and invasion of SMMC-7721 cells. It may be related with the down-regulation of p-STAT3 and MMP-2.

摘要

目的

分析白细胞介素-37（IL-37）对SMMC-7721肝癌细胞增殖、迁移和侵袭的影响及其相关分子机制。

方法

将体外培养的SMMC-7721细胞分为（50、100、200、500）ng/mL重组人IL-37（rhIL-37）处理组和对照组。采用CCK-8法检测细胞增殖能力。采用划痕实验观察细胞迁移能力。采用Transwell实验检测细胞侵袭能力。运用蛋白质印迹法检测信号转导和转录激活因子3（STAT3）、磷酸化STAT3（p-STAT3）和基质金属蛋白酶-2（MMP-2）的水平。