[Inhibitory effect of 2'-hydroxyflavanone on proliferation, invasion and migration of bladder cancer cells in vitro via blocking AKT/STAT3 signaling pathway].

OBJECTIVE

To study the effect of 2'-hydroxyflavanone on proliferation, invasion and migration of bladder cancer cells and its mechanism.

METHODS

MTT assay was used to detect the effect of 2'-hydroxyflavanone on proliferation of bladdercancer cells, and the proliferation inhibition rate was calculated. Wound healing assay and Transwell (TM); assay were conducted to study the effect of 2'-hydroxyflavanone on migration and invasion of bladder cancer cells, respectively. Western blotting was performed to investigate the change of migration-associated proteins after 2'-hydroxyflavanone treatment.

RESULTS

2'-hydroxyflavanone inhibited the proliferation of bladder cancer cells (5637, T24, UMUC-3, 253J) in a time- and dose- dependent manner. The migration and invasion abilities of T24 cells were significantly reduced by 2'-hydroxyflavanone treatment (P<0.05), which was accompanied by the down-regulation of MMP-2, MMP-9, p-AKT and p-STAT3 protein expressions.

CONCLUSION

2'-hydroxyflavanone can effectively inhibit bladder cancer cell proliferation, invasion and migration, in which the inhibition of AKT/STAT3 signaling pathway may play an important role.