Wang Yan-Su, Shi Wei, Huang Lin-Ting, Ding Cheng-Long, Dai Chuan-Chao
Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Jiangsu Nanjing, China.
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Jiangsu Nanjing, China.
Anim Sci J. 2016 Apr;87(4):525-35. doi: 10.1111/asj.12449. Epub 2015 Oct 1.
Lactic acid bacteria (LAB) are suitable for rice straw silage fermentation, but have been studied rarely, and rice straw as raw material for ensiling is difficult because of its disadvantages, such as low nutrition for microbial activities and low abundances of natural populations of LAB. So we investigated the effect of application of LAB and chemical additives on the fermentation quality and microbial community of wilted rice straw silage. Treatment with chemical additives increased the concentrations of crude protein (CP), water soluble carbohydrate (WSC), acetic acid and lactic acid, reduced the concentrations of acid detergent fiber (ADF) and neutral detergent fiber (NDF), but did not effectively inhibit the growth of spoilage organisms. Inoculation with LABs did not improve the nutritional value of the silage because of poor growth of LABs in wilted rice straw. Inoculation with LAB and addition of chemical materials improved the quality of silage similar to the effects of addition of chemical materials alone. Growth of aerobic and facultatively anaerobic bacteria was inhibited by this mixed treatment and the LAB gradually dominated the microbial community. In summary, the fermentation quality of wilted rice straw silage had improved by addition of LAB and chemical materials.
乳酸菌(LAB)适用于稻草青贮发酵,但相关研究较少,且稻草作为青贮原料存在劣势,如微生物活动的营养成分低以及乳酸菌自然种群数量少,因此难以青贮。所以我们研究了添加乳酸菌和化学添加剂对萎蔫稻草青贮发酵品质和微生物群落的影响。添加化学添加剂处理提高了粗蛋白(CP)、水溶性碳水化合物(WSC)、乙酸和乳酸的浓度,降低了酸性洗涤纤维(ADF)和中性洗涤纤维(NDF)的浓度,但未能有效抑制腐败微生物的生长。由于乳酸菌在萎蔫稻草中生长不佳,接种乳酸菌并未提高青贮饲料的营养价值。接种乳酸菌并添加化学物质对青贮品质的改善效果与单独添加化学物质相似。这种混合处理抑制了需氧菌和兼性厌氧菌的生长,乳酸菌逐渐在微生物群落中占据主导地位。总之,添加乳酸菌和化学物质改善了萎蔫稻草青贮的发酵品质。