Babaeinasab Y, Rouzbehan Y, Fazaeli H, Rezaei J
J Anim Sci. 2015 Sep;93(9):4377-86. doi: 10.2527/jas.2015-9082.
The aim of this study was to determine the effect of molasses and lactic acid bacteria (LAB) on the chemical composition, silage fermentation characteristics, and in vitro ruminal fermentation parameters of an ensiled potato-wheat straw mixture in a completely randomized design with 4 replicates. Wheat straw was harvested at full maturity and potato tuber when the leaves turned yellowish. The potato-wheat straw (57:43 ratio, DM basis) mixture was treated with molasses, LAB, or a combination. Lalsil Fresh LB (Lallemand, France; containing NCIMB 40788) or Lalsil MS01 (Lallemand, France; containing MA18/5U and MA126/4U) were each applied at a rate of 3 × 10 cfu/g of fresh material. Treatments were mixed potato-wheat straw silage (PWSS) without additive, PWSS inoculated with Lalsil Fresh LB, PWSS inoculated with Lalsil MS01, PWSS + 5% molasses, PWSS inoculated with Lalsil Fresh LB + 5% molasses, PWSS inoculated with Lalsil MS01 + 5% molasses, and corn silage (CS). The compaction densities of PWSS treatments and CS were approximately 850 and 980 kg wet matter/m, respectively. After anaerobic storage for 90 d, chemical composition, silage fermentation characteristics, in vitro gas production (GP), estimated OM disappearance (OMD), ammonia-N, VFA, microbial CP (MCP) production, and cellulolytic bacteria count were determined. Compared to CS, PWSS had greater ( < 0.001) values of DM, ADL, water-soluble carbohydrates, pH, and ammonia-N but lower ( < 0.05) values of CP, ash free-NDF (NDFom), ash, nitrate, and lactic, acetic, propionic, and butyric acids concentrations. When PWSS was treated with molasses, LAB, or both, the contents of CP and lactic and acetic acids increased, whereas NDFom, ammonia-N, and butyric acid decreased ( < 0.05). Based on in vitro ruminal experiments, PWSS had greater ( < 0.05) values of GP, OMD, and MCP but lower ( < 0.05) VFA and acetic acid compared to CS. With adding molasses alone or in combination with LAB inoculants to PWSS, the values of GP, OMD, MCP, cellulolytic bacteria population, VFA, and propionic acid increased ( < 0.05), whereas the acetic acid to propionic acid ratio decreased ( < 0.05). Overall, ensiling potato with wheat straw at a 57:43 ratio DM basis was possible; nevertheless, the fermentation quality of PWSS was lesser than that of CS. However, addition of molasses and molasses + LAB improved fermentation quality of PWSS.
本研究的目的是采用完全随机设计(4次重复),确定糖蜜和乳酸菌(LAB)对青贮马铃薯 - 小麦秸秆混合物的化学成分、青贮发酵特性及体外瘤胃发酵参数的影响。小麦秸秆在完全成熟时收割,马铃薯块茎在叶片变黄时收获。将马铃薯 - 小麦秸秆混合物(干物质基础比例为57:43)用糖蜜、乳酸菌或二者组合进行处理。法国乐斯福公司的Lalsil Fresh LB(含NCIMB 40788)或Lalsil MS01(含MA18/5U和MA126/4U)均按3×10 cfu/g新鲜物料的用量施用。处理组包括不添加添加剂的混合马铃薯 - 小麦秸秆青贮料(PWSS)、接种Lalsil Fresh LB的PWSS、接种Lalsil MS01的PWSS、PWSS + 5%糖蜜、接种Lalsil Fresh LB + 5%糖蜜的PWSS、接种Lalsil MS01 + 5%糖蜜的PWSS以及玉米青贮料(CS)。PWSS处理组和CS的压实密度分别约为850和980 kg湿物质/m³。厌氧储存90天后,测定其化学成分、青贮发酵特性、体外产气(GP)、估计有机物消失率(OMD)、氨态氮、挥发性脂肪酸(VFA)、微生物粗蛋白(MCP)产量以及纤维素分解菌数量。与CS相比,PWSS的干物质、酸性洗涤木质素、水溶性碳水化合物、pH值和氨态氮含量更高(P < 0.001),但粗蛋白、无灰中性洗涤纤维(NDFom)、灰分、硝酸盐以及乳酸、乙酸、丙酸和丁酸浓度更低(P < 0.05)。当PWSS用糖蜜、乳酸菌或二者同时处理时,粗蛋白以及乳酸和乙酸含量增加,而NDFom、氨态氮和丁酸含量降低(P < 0.05)。基于体外瘤胃实验,与CS相比,PWSS的GP、OMD和MCP值更高(P < 0.05),但VFA和乙酸含量更低(P < 0.05)。单独向PWSS添加糖蜜或与乳酸菌接种剂联合添加时,GP、OMD、MCP、纤维素分解菌数量、VFA和丙酸含量增加(P < 0.05),而乙酸与丙酸的比例降低(P < 0.05)。总体而言,以干物质基础比例57:43将马铃薯与小麦秸秆青贮是可行的;然而,PWSS的发酵质量低于CS。不过,添加糖蜜以及糖蜜 + 乳酸菌可提高PWSS的发酵质量。