Tashiro Ayumu, Zhao Chunmei, Suh Hoonkyo, Gage Fred H
Cold Spring Harb Protoc. 2015 Oct 1;2015(10):932-3. doi: 10.1101/pdb.prot086389.
This protocol describes the harvesting of brain tissue from mice that have had the retroviral vector CAG-GFP injected into the dentate gyrus. Brain tissue from these mice is dissected, the tissue is fixed, and the sections are prepared. The fixed sections are imaged using fluorescent confocal microscopy, and newborn granule cells containing GFP are visualized and are characterized.
本方案描述了从已将逆转录病毒载体CAG-GFP注射到齿状回的小鼠中获取脑组织的过程。对这些小鼠的脑组织进行解剖、固定并制备切片。使用荧光共聚焦显微镜对固定后的切片进行成像，观察并鉴定含有绿色荧光蛋白（GFP）的新生颗粒细胞。
