一种适用于高通量筛选和联合测试的384孔抗菌检测方法的建立与验证。
Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing.
作者信息
Mishra Arti, Dobritsa Svetlana V, Crouch Marie-Laure, Rabenstein John, Lee Joycelyn Xiang Yi, Dhakshinamoorthy Saravanakumar
机构信息
Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore.
Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore; Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
出版信息
J Microbiol Methods. 2015 Nov;118:173-5. doi: 10.1016/j.mimet.2015.09.019. Epub 2015 Oct 1.
A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.
本文描述了一种适用于高通量筛选和联合测试的基于384孔板的抗菌检测方法。该检测方法使用100 - 500nL的测试化合物，可耐受高达2.5%的二甲基亚砜浓度。它可用于筛选化合物文库，并测试抗生素、小分子和天然产物提取物的组合/协同/拮抗作用。
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