基于微量滴定板的抗菌测定法，以刃天青作为细胞生长指标及其在植物化学物质体外抗菌筛选中的应用
Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals.
作者信息
Sarker Satyajit D, Nahar Lutfun, Kumarasamy Yashodharan
机构信息
School of Biomedical Sciences, University of Ulster at Coleraine, Cromore Road, Coleraine BT52 1SA, Co. Londonderry, Northern Ireland, UK.
出版信息
Methods. 2007 Aug;42(4):321-4. doi: 10.1016/j.ymeth.2007.01.006.
The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.
2000年，德拉蒙德（Drummond）和韦（Waigh）描述了利用微量滴定板的刃天青试验，该试验已被改进，以在测定天然产物（包括粗提物、色谱馏分或纯化化合物）对各种细菌菌株的最低抑菌浓度（MIC）值时获得更高的准确性。这种改进的刃天青方法简单、灵敏、快速、稳健且可靠，可成功用于评估天然产物的抗菌特性。
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