在实验性糖尿病中，维生素C和维生素E（抗坏血酸盐/α-生育酚）在空肠中提供协同神经保护作用。

Vitamins C and E (ascorbate/α-tocopherol) provide synergistic neuroprotection in the jejunum in experimental diabetes.

作者信息

Tashima Cristiano Massao, Hermes-Uliana Catchia, Perles Juliana Vanessa Colombo Martins, de Miranda Neto Marcilio Hubner, Zanoni Jacqueline Nelisis

机构信息

Department of Health and Education, Universidade Estadual do Norte do Paraná, 86360-000 Paraná, Brazil.

Universidade Federal de Mato Grosso do Sul, 79400-000 Coxim, Mato Grosso do Sul, Brazil.

出版信息

Pathophysiology. 2015 Dec;22(4):241-8. doi: 10.1016/j.pathophys.2015.09.003. Epub 2015 Sep 28.

DOI:10.1016/j.pathophys.2015.09.003

PMID:26433445

Abstract

The present study evaluated the synergistic effects of the association of ascorbic acid and α-tocopherol on myenteric in the jejunum of diabetic rats. The rats were randomly divided into four equal groups: untreated normoglycemic (UC), untreated diabetic (UD), ascorbic acid and α-tocopherol-treated normoglycemic (CAE) and ascorbic acid and α-tocopherol-treated diabetic (DAE). The rats from the CAE and DAE group received supplementation with ascorbic acid (1g/L in water) and α-tocopherol (1% in chow). At 210-days-old, the animals were sacrified and their jejunum was collected and submitted to immunohistochemistry. Quantitative and/or morphometric analysis were performed. Supplementation with ascorbic acid and α-tocopherol prevented the cell loss of myenteric neurons expressing HuC/D and TrkA in an equivalent proportion. We also observed a reduction of the CGRP nerve fiber varicosities and the prevention of the increased cell body size of submucosal VIP neurons (p<0.05). The association of ascorbic acid and α-tocopherol reduced the deleterious effects of diabetes promoting protection on the enteric neurons.

摘要

本研究评估了抗坏血酸和α-生育酚联合使用对糖尿病大鼠空肠肌间神经丛的协同作用。大鼠被随机分为四组，每组数量相等：未治疗的正常血糖组（UC）、未治疗的糖尿病组（UD）、抗坏血酸和α-生育酚治疗的正常血糖组（CAE）以及抗坏血酸和α-生育酚治疗的糖尿病组（DAE）。CAE组和DAE组的大鼠接受了抗坏血酸（水中1g/L）和α-生育酚（饲料中1%）的补充。在210日龄时，处死动物并收集其空肠，进行免疫组织化学分析。进行了定量和/或形态计量分析。补充抗坏血酸和α-生育酚以相同比例防止了表达HuC/D和TrkA的肌间神经元细胞丢失。我们还观察到降钙素基因相关肽（CGRP）神经纤维膨体减少，以及黏膜下血管活性肠肽（VIP）神经元胞体增大得到预防（p<0.05）。抗坏血酸和α-生育酚联合使用减少了糖尿病的有害影响，对肠神经元起到保护作用。