Tontrong Sopa, Khonyoung Supada, Jakmunee Jaroon
Department of Chemistry, and Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Chemistry, and Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Food Chem. 2012 May 1;132(1):624-9. doi: 10.1016/j.foodchem.2011.10.100. Epub 2011 Nov 7.
A flow injection (FI) spectrophotometric method with using natural reagent extracted from Morinda citrifolia root has been developed for determination of aluminium. The extract contained anthraquinone compounds which could react with Al(3+) to form reddish complexes which had maximum absorption wavelength at 499.0nm. The extract could be used as a reagent in FI system without further purification to obtain pure compound. A sensitive method for determination of aluminium in concentration range of 0.1-1.0mgL(-1), with detection limit of 0.05mgL(-1) was achieved. Relative standard deviations of 1.2% and 1.7% were obtained for the determination of 0.1 and 0.6mgL(-1) Al(3+) (n=11). Sample throughput of 35h(-1) was achieved with the consumption of 3mL each of carrier and reagent solutions per injection. The developed method was successfully applied to tea samples, validated by the FAAS standard method. The method is simple, fast, economical and could be classified as a greener analytical method.
建立了一种流动注射（FI）分光光度法，该方法使用从巴戟天根中提取的天然试剂来测定铝。提取物中含有蒽醌类化合物，其可与Al(3+)反应形成红色络合物，该络合物在499.0nm处有最大吸收波长。该提取物无需进一步纯化以获得纯化合物即可用作FI系统中的试剂。实现了一种在0.1 - 1.0mgL(-1)浓度范围内测定铝的灵敏方法，检测限为0.05mgL(-1)。测定0.1和0.6mgL(-1) Al(3+)（n = 11）时，相对标准偏差分别为1.2%和1.7%。每次进样时，载流和试剂溶液的消耗量均为3mL，实现了35h(-1)的样品通量。所建立的方法成功应用于茶叶样品，并通过火焰原子吸收光谱法（FAAS）标准方法进行了验证。该方法简单、快速、经济，可归类为一种更环保的分析方法。
