Martins Joana, Esteves Cristina, Limpo-Faria Ana, Barros Paulo, Ribeiro Natália, Simões Tomás, Correia Manuela, Delerue-Matos Cristina
IVDP, Instituto dos Vinhos do Douro e do Porto, Rua de Ferreira Borges, 27, 4050-253 Porto, Portugal; REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal.
IVDP, Instituto dos Vinhos do Douro e do Porto, Rua de Ferreira Borges, 27, 4050-253 Porto, Portugal.
Food Chem. 2012 May 1;132(1):630-6. doi: 10.1016/j.foodchem.2011.10.102. Epub 2011 Nov 7.
A multiresidue gas chromatographic method for the determination of six fungicides (captan, chlorthalonil, folpet, iprodione, procymidone and vinclozolin) and one acaricide (dicofol) in still and fortified wines was developed. Solid-phase microextraction (SPME) was chosen for the extraction of the compounds from the studied matrices and tandem mass spectrometry (MS/MS) detection was used. The extraction consists in a solvent free and automated procedure and the detection is highly sensitive and selective. Good linearity was obtained with correlation coefficients of regression (R(2))>0.99 for all the compounds. Satisfactory results of repeatability and intermediate precision were obtained for most of the analytes (RSD⩽20%). Recoveries from spiked wine ranged from 80.1% to 112.0%. Limits of quantification (LOQs) were considerably below the proposed maximum residue limits (MRLs) for these compounds in grapes and below the suggested limits for wine (MRLs/10), with the exception of captan.
建立了一种多残留气相色谱法，用于测定静止葡萄酒和加标葡萄酒中的六种杀菌剂（克菌丹、百菌清、灭菌丹、异菌脲、腐霉利和乙烯菌核利）和一种杀螨剂（三氯杀螨醇）。采用固相微萃取（SPME）从研究基质中萃取化合物，并使用串联质谱（MS/MS）检测。萃取采用无溶剂自动化程序，检测具有高灵敏度和选择性。所有化合物的回归相关系数（R(2)）>0.99，线性良好。大多数分析物的重复性和中间精密度结果令人满意（RSD⩽20%）。加标葡萄酒的回收率在80.1%至112.0%之间。除克菌丹外，定量限（LOQ）远低于葡萄中这些化合物建议的最大残留限量（MRL）以及葡萄酒建议限量（MRL/10）。
