ORF119基因缺失对羊口疮病毒复制和毒力的影响。
Effect of ORF119 gene deletion on the replication and virulence of orf virus.
作者信息
Qiao J, Yang H B, Peng Y L, Meng Q L, Chen C, Ma Y, Xie K, Liu T L, Cai X P, Chen C F
出版信息
Acta Virol. 2015 Sep;59(3):257-64. doi: 10.4149/av_2015_03_257.
Orf is a severe infectious disease of sheep and goats caused by orf virus (ORFV). To investigate the role of ORF119 gene of ORFV, we constructed ORFV with deleted ORF119 gene and LacZ as reporter gene (ORFV-Δ119-LacZ) via homologous recombination. The results showed that wild-type ORF-SHZ1 and ORFV-Δ119-LacZ deletion viruses replicated in Vero cells to similar titers. Relative transcriptional levels of virulence genes OVIFNR, GIF, VEGF and VIL-10 of ORFV-Δ119-LacZ deletion virus were slightly but not significantly lower after 24 hr compared with the wtORF-SHZ1 virus. In vivo experiments showed that 2-month-old lambs inoculated with ORFV-Δ119-LacZ deletion virus exhibited a similar total clinical score compared with those inoculated with wtORF-SHZ1 virus. Based on these results, we conclude that deletion of the ORF119 gene has no significant effect on ORFV replication and virulence.
羊口疮是由羊口疮病毒（ORFV）引起的绵羊和山羊的一种严重传染病。为了研究ORFV的ORF119基因的作用，我们通过同源重组构建了缺失ORF119基因并以LacZ作为报告基因的ORFV（ORFV-Δ119-LacZ）。结果表明，野生型ORF-SHZ1和ORFV-Δ119-LacZ缺失病毒在Vero细胞中复制至相似滴度。与wtORF-SHZ1病毒相比，ORFV-Δ119-LacZ缺失病毒的毒力基因OVIFNR、GIF、VEGF和VIL-10在24小时后的相对转录水平略有降低，但不显著。体内实验表明，接种ORFV-Δ119-LacZ缺失病毒的2月龄羔羊与接种wtORF-SHZ1病毒的羔羊相比，总临床评分相似。基于这些结果，我们得出结论，ORF119基因的缺失对ORFV的复制和毒力没有显著影响。
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