Gupta Abhishek, Kumar Rahul, Sahu Vishal, Agnihotri Vertica, Singh Amrendra Pratap, Bhasker Suman, Dey Sharmistha
Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Department of Radiation Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Biochem Biophys Res Commun. 2015 Nov 13;467(2):248-53. doi: 10.1016/j.bbrc.2015.09.181. Epub 2015 Oct 3.
Head and neck squamous cell carcinoma (HNSCC) is the major health concern in Indian population. Despite of advanced treatment the mortality rate for this disease has not been improved very much. Current research focused on development of protein marker for the diagnosis and prognosis of HNSCC. The case control study was performed with 125 HNSCC patients and 104 control cases. The level of p50 and IκBα proteins in serum were evaluated at pre and post therapy by label free real time surface plasmon resonance (SPR) and western blot analysis. The serum p50 concentration were significantly (P < 0.0001) higher at the time of diagnosis i.e. pre therapy (Mean ± SD = 27.06 ± 4.88 ng/μl) as compared to controls (Mean ± SD = 16.96 ± 4.04 ng/μl) while it decline at post therapy (Mean ± SD = 21.01 ± 4.98 ng/μl). Similarly, the concentration of IκBα protein in serum were slightly higher at pre therapy (Mean ± SD = 8.33 ± 1.85 ng/μl) as compared to controls (Mean ± SD = 7.27 ± 1.84 ng/μl) and declined at post therapy (Mean ± SD = 7.09 ± 1.24 ng/μl). The level of p50 was also high at the early stage of the disease. The specificity and sensitivity of p50 proteins obtained from ROC analysis revealed the potentiality to be diagnostic protein marker for HNSCC for its accuracy in the study cohort.
头颈部鳞状细胞癌（HNSCC）是印度人群主要的健康问题。尽管有先进的治疗方法，但这种疾病的死亡率并没有得到很大改善。目前的研究集中在开发用于HNSCC诊断和预后的蛋白质标志物。对125例HNSCC患者和104例对照病例进行了病例对照研究。通过无标记实时表面等离子体共振（SPR）和蛋白质印迹分析，在治疗前和治疗后评估血清中p50和IκBα蛋白的水平。与对照组（均值±标准差=16.96±4.04 ng/μl）相比，诊断时即治疗前血清p50浓度显著更高（P<0.0001）（均值±标准差=27.06±4.88 ng/μl），而治疗后下降（均值±标准差=21.01±4.98 ng/μl）。同样，血清中IκBα蛋白浓度在治疗前略高于对照组（均值±标准差=8.33±1.85 ng/μl）（均值±标准差=7.27±1.84 ng/μl），治疗后下降（均值±标准差=7.09±1.24 ng/μl）。在疾病早期p50水平也较高。从ROC分析获得的p50蛋白的特异性和敏感性显示，因其在研究队列中的准确性，有潜力成为HNSCC的诊断蛋白质标志物。
