Choi Hee-Soo, Lee Choong-Hyun
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Korea.
J Vet Sci. 2016 Jun 30;17(2):137-44. doi: 10.4142/jvs.2016.17.2.137.
Hippocalcin participates in the maintenance of neuronal calcium homeostasis. In the present study, we examined the time-course changes of neuronal degeneration and hippocalcin protein level in the mouse hippocampus following pilocarpine-induced status epilepticus (SE). Marked neuronal degeneration was observed in the hippocampus after SE in a time-dependent manner, although neuronal degeneration differed according to the hippocampal subregions. Almost no hippocalcin immunoreactivity was detected in the pyramidal neurons of the cornu ammonis 1 (CA1) region from 6 h after SE. However, many pyramidal neurons in the CA2 region showed hippocalcin immunoreactivity until 24 h after SE. In the CA3 region, only a few hippocalcin immunoreactive cells were observed at 12 h after SE, and almost no hippocalcin immunoreactivity was observed in the pyramidal neurons from 24 h after SE. Hippocalcin immunoreactivity in the polymorphic cells of the dentate gyrus was markedly decreased from 6 h after SE. In addition, hippocalcin protein level in the hippocampus began to decrease from 6 h after SE, and was significantly decreased at 24 h and 48 h after pilocarpine-induced SE. These results indicate that marked reduction of hippocalcin level may be closely related to neuronal degeneration in the hippocampus following pilocarpine-induced SE.
海马钙结合蛋白参与神经元钙稳态的维持。在本研究中，我们检测了匹鲁卡品诱导的癫痫持续状态（SE）后小鼠海马中神经元变性和海马钙结合蛋白水平的时间进程变化。SE后海马中观察到明显的神经元变性，且呈时间依赖性，尽管不同海马亚区的神经元变性有所不同。SE后6小时起，在海马1区（CA1）的锥体细胞中几乎检测不到海马钙结合蛋白免疫反应性。然而，CA2区的许多锥体细胞在SE后24小时内均显示出海马钙结合蛋白免疫反应性。在CA3区，SE后12小时仅观察到少数海马钙结合蛋白免疫反应性细胞，SE后24小时起在锥体细胞中几乎检测不到海马钙结合蛋白免疫反应性。齿状回多形细胞中的海马钙结合蛋白免疫反应性从SE后6小时起显著降低。此外，海马中的海马钙结合蛋白水平从SE后6小时开始下降，在匹鲁卡品诱导的SE后24小时和48小时显著降低。这些结果表明，海马钙结合蛋白水平的显著降低可能与匹鲁卡品诱导的SE后海马中的神经元变性密切相关。
