Qin L J, Zhang T, Jia Y S, Zhang Y B, Zhang Y X, Wang H T
School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China
School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China.
Genet Mol Res. 2015 Oct 2;14(4):11771-9. doi: 10.4238/2015.October.2.11.
Thermotherapy has been proven to be effective for the treatment of various tumors, including glioma. We determined whether tumor necrosis factor-alpha (TNF-α) is involved in the regulation of the biological processes of glioma development. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry were used to investigate the levels of TNF-α mRNA and heat shock factor-1 (HSF1) protein, respectively, in glioma cells. Radioimmunoassay was used to dynamically monitor the contents of TNF-α in the nutrient fluid of C6 cells after thermotherapy treatment. Crystal violet staining was used to determine glioma invasiveness. The most obvious increases in HSF1 protein and TNF-α mRNA in C6 cells were observed at 30 and 60 min after thermotherapy, respectively. In addition, the radioactivity of TNF-α in the culture fluid of the C6 cells reached a peak after 120 min of thermotherapy. In addition, glioma invasiveness decreased and the concentration of TNF-α reached a maximum after 120 min of thermotherapy. Our results show that the decrease in thermotherapy-mediated glioma invasiveness is due to the accelerated release of TNF-α, which could promote the release of HSF1 from neurospongioma cells.
热疗法已被证明对包括神经胶质瘤在内的各种肿瘤的治疗有效。我们确定肿瘤坏死因子-α（TNF-α）是否参与神经胶质瘤发生发展生物学过程的调控。分别采用逆转录-聚合酶链反应（RT-PCR）和免疫细胞化学方法检测神经胶质瘤细胞中TNF-α mRNA水平和热休克因子-1（HSF1）蛋白水平。采用放射免疫分析法动态监测热疗处理后C6细胞培养液中TNF-α含量。采用结晶紫染色法测定神经胶质瘤的侵袭性。热疗后30分钟和60分钟时，C6细胞中HSF1蛋白和TNF-α mRNA的增加最为明显。此外，热疗120分钟后，C6细胞培养液中TNF-α的放射性达到峰值。另外，热疗120分钟后神经胶质瘤侵袭性降低，TNF-α浓度达到最大值。我们的结果表明，热疗介导的神经胶质瘤侵袭性降低是由于TNF-α加速释放所致，TNF-α可促进神经胶质瘤细胞释放HSF1。
