Li Zhe, Zhang Yuting, Chen Shixi, Chen Wei, Hong Wanshu
a College of Ocean and Earth Sciences, Xiamen University , Xiamen , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3812-4. doi: 10.3109/19401736.2015.1082089. Epub 2015 Oct 5.
In this paper, the complete mitochondrial genome sequence of Scartelaos gigas was firstly determined. The circular genome (16 717 bp) comprises 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The overall base composition of S. gigas is 28.9% for C, 28.3% for A, 26.4% for T, 16.4% for G, with a slight A + T bias of 54.7%. In the control region, the termination-associated sequence and conserved sequence block domains were found, but the tandem repeat structure was not found. It has the typical vertebrate mitochondrial gene arrangement. The phylogenic analysis using the Neighbor-Joining method showed that the fishes belonging to Gobiidae, Odontoburidae, and Eleotridae formed three branches grouped with other fishes into one clade which separated from the mammals. We hope that the results from the present study will provide useful molecular information for the further studies on genetic structure and demographic history of S. gigas.
本文首次测定了大弹涂鱼的完整线粒体基因组序列。该环状基因组（16717 bp）包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区。大弹涂鱼的总体碱基组成为：C占28.9%，A占28.3%，T占26.4%，G占16.4%，A+T略有偏向，为54.7%。在控制区，发现了终止相关序列和保守序列块结构域，但未发现串联重复结构。它具有典型的脊椎动物线粒体基因排列。使用邻接法进行的系统发育分析表明，属于虾虎鱼科、齿塘鳢科和塘鳢科的鱼类形成三个分支，与其他鱼类一起聚为一个进化枝，该进化枝与哺乳动物分开。我们希望本研究结果能为大弹涂鱼遗传结构和种群历史的进一步研究提供有用的分子信息。
