Liu Li Na, Wang Han, Jiang Peng, Wang Man, Xu Ming Guo, Wang Li Fu, Qi Xin, Zhang Xi, Cui Jing, Wang Zhong Quan
Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China.
Parasitol Res. 2015 Dec;114(12):4737-42. doi: 10.1007/s00436-015-4758-7. Epub 2015 Oct 6.
The Spirometra erinacei casein kinase I (SeCKI) gene was cloned and expressed in Escherichia coli, and its characteristics were investigated in this study. The recombinant SeCP protein (rSeCKI) was purified. The vaccination of mice with rSeCKI induced the Th1/Th2-mixed type of immune response with Th2 predominant (high levels of IgG1). Western blotting analysis showed that rSeCP was recognized by the sera of plerocercoid-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude antigens. Transcription and expression of SeCP was observed at the plerocercoid and adult stages of S. erinacei. Immunolocalization identified SeCKI in the tegument and parenchymal tissues of plerocercoids and in the teguments of adults. SeCKI appeared to be essential indispensable for the S. erinacei development and survival in host, but its biological functions need to be further investigated.
本研究克隆了猬迭宫绦虫酪蛋白激酶I（SeCKI）基因并在大肠杆菌中进行表达，同时对其特性进行了研究。纯化了重组SeCP蛋白（rSeCKI）。用rSeCKI对小鼠进行免疫接种可诱导以Th2为主导（IgG1水平高）的Th1/Th2混合型免疫反应。蛋白质印迹分析表明，rSeCP可被裂头蚴感染小鼠的血清识别，抗rSeCP血清可识别裂头蚴粗抗原中的天然SeCP蛋白。在猬迭宫绦虫的裂头蚴和成虫阶段均观察到SeCP的转录和表达。免疫定位在裂头蚴的皮层和实质组织以及成虫的皮层中鉴定出SeCKI。SeCKI似乎对猬迭宫绦虫在宿主体内的发育和存活至关重要，但其生物学功能有待进一步研究。
