Iftime Dumitrita, Jasyk Martin, Kulik Andreas, Imhoff Johannes F, Stegmann Evi, Wohlleben Wolfgang, Süssmuth Roderich D, Weber Tilmann
Interfakultäres Institut für Mikrobiologie und Infektionsmedizin Tübingen, Mikrobiologie/Biotechnologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 10623, Berlin, Germany.
Chembiochem. 2015 Dec;16(18):2615-23. doi: 10.1002/cbic.201500377. Epub 2015 Nov 6.
Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.
羊毛硫肽是核糖体合成并经翻译后修饰的微生物次级代谢产物。在此，我们报告了从链霉菌属Tü 365中鉴定和分离出链霉菌素，它是IV类羊毛硫肽的一个新成员。在羊毛硫肽合成酶基因stcL上游插入组成型ermE*启动子可导致肽的产生。链霉菌素基因簇在天蓝色链霉菌M1146和M1152中进行了异源表达，产量分别提高了3.5倍和5.5倍。通过高分辨率MS/MS分析确定了链霉菌素的结构和环拓扑结构。链霉菌素含有四个大环，一个羊毛硫氨酸和三个甲基羊毛硫氨酸残基。据我们所知，这是关于以制备量分离IV类羊毛硫肽以及IV类羊毛硫肽基因簇成功异源表达的首次报道。
