Ogino Tomoaki
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine.
Uirusu. 2014;64(2):165-78. doi: 10.2222/jsv.64.165.
Non-segmented negative strand RNA viruses belonging to the Mononegavirales order possess RNA-dependent RNA polymerase L proteins within viral particles. The L protein is a multifunctional enzyme catalyzing viral RNA synthesis and processing (i.e., mRNA capping, cap methylation, and polyadenylation). Using vesicular stomatitis virus (VSV) as a prototypic model virus, we have shown that the L protein catalyzes the unconventional mRNA capping reaction, which is strikingly different from the eukaryotic reaction. Furthermore, co-transcriptional pre-mRNA capping with the VSV L protein was found to be required for accurate stop?start transcription to synthesize full-length mRNAs in vitro and virus propagation in host cells. This article provides a review of historical and present studies leading to the elucidation of the molecular mechanism of VSV mRNA capping.
属于单股负链RNA病毒目（Mononegavirales）的非节段性负链RNA病毒在病毒颗粒中含有依赖RNA的RNA聚合酶L蛋白。L蛋白是一种多功能酶，催化病毒RNA的合成和加工（即mRNA加帽、帽甲基化和多聚腺苷酸化）。我们以水泡性口炎病毒（VSV）作为典型的模型病毒，已证明L蛋白催化非常规的mRNA加帽反应，这与真核反应显著不同。此外，发现与VSV L蛋白进行共转录前体mRNA加帽对于体外准确的终止-起始转录以合成全长mRNA以及在宿主细胞中的病毒传播是必需的。本文综述了导致阐明VSV mRNA加帽分子机制的历史和当前研究。
