Huang Yunyi, Wu Xuling, Guo Jing, Yuan Jinxian
a Department of Neurology, The Second Affiliated Hospital, Chongqing Medical University , Yangmei Chen , China.
Int J Neurosci. 2016 Sep;126(9):786-96. doi: 10.3109/00207454.2015.1062003. Epub 2015 Oct 6.
Myocyte-specific enhancer binding factor 2A (MEF2A) is a multifunctional nuclear protein that regulates synaptogenesis, dendritic morphogenesis, and neuronal survival. This study aimed to investigate the expression pattern of MEF2A in epileptogenic processes. MEF2A expression was detected in 20 temporal neocortex tissue samples from patients with temporal lobe epilepsy (TLE) and 20 samples from trauma patients without epilepsy by real-time quantitative polymerase chain reaction, immunohistochemistry, double-label immunofluorescent staining, and western blot analysis. In addition, the expression patterns of MEF2A in the hippocampus and adjacent cortex of a lithium-pilocarpine-induced TLE rat model and control rats were examined. MEF2A was found to be expressed in the nuclei of neurons but not in the dendrites of neurons and astrocytes. MEF2A expression was significantly downregulated in temporal neocortex of humans and rats with TLE compared to the control groups. In addition, in the lithium-pilocarpine-induced TLE model, MEF2A expression dynamically decreased within 2 months. Taken together, these data suggest that MEF2A is involved in the pathogenesis of TLE.
心肌细胞特异性增强子结合因子2A（MEF2A）是一种多功能核蛋白，可调节突触形成、树突形态发生和神经元存活。本研究旨在探讨MEF2A在癫痫发生过程中的表达模式。通过实时定量聚合酶链反应、免疫组织化学、双标免疫荧光染色和蛋白质印迹分析，检测了20例颞叶癫痫（TLE）患者的颞叶新皮质组织样本以及20例无癫痫的创伤患者样本中MEF2A的表达。此外，还检测了锂-匹罗卡品诱导的TLE大鼠模型和对照大鼠海马及相邻皮质中MEF2A的表达模式。结果发现MEF2A在神经元细胞核中表达，但在神经元和星形胶质细胞的树突中不表达。与对照组相比，TLE患者和大鼠颞叶新皮质中MEF2A的表达明显下调。此外，在锂-匹罗卡品诱导的TLE模型中，MEF2A的表达在2个月内动态下降。综上所述，这些数据表明MEF2A参与了TLE的发病机制。
