Lupu Daniel S, Cheatham Carol L, Corbin Karen D, Niculescu Mihai D
Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, NC 28081, USA; Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, NC 28081, USA; Department of Psychology & Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Nutr Res. 2015 Nov;35(11):939-47. doi: 10.1016/j.nutres.2015.09.004. Epub 2015 Sep 10.
Polyunsaturated fatty acid metabolism in toddlers is regulated by a complex network of interacting factors. The contribution of maternal genetic and epigenetic makeup to this milieu is not well understood. In a cohort of mothers and toddlers 16 months of age (n = 65 mother-child pairs), we investigated the association between maternal genetic and epigenetic fatty acid desaturase 2 (FADS2) profiles and toddlers' n-6 and n-3 fatty acid metabolism. FADS2 rs174575 variation and DNA methylation status were interrogated in mothers and toddlers, as well as food intake and plasma fatty acid concentrations in toddlers. A multivariate fit model indicated that maternal rs174575 genotype, combined with DNA methylation, can predict α-linolenic acid plasma concentration in all toddlers and arachidonic acid concentrations in boys. Arachidonic acid intake was predictive for its plasma concentration in girls, whereas intake of 3 major n-3 species (eicosapentaenoic, docosapentaenoic, and docosahexaenoic acids) were predictive for their plasma concentrations in boys. FADS2 genotype and DNA methylation in toddlers were not related to plasma concentrations or food intakes, except for CpG8 methylation. Maternal FADS2 methylation was a predictor for the boys' α-linolenic acid intakes. This exploratory study suggests that maternal FADS2 genetic and epigenetic status could be related to toddlers' polyunsaturated fatty acid metabolism.
幼儿体内的多不饱和脂肪酸代谢受复杂的相互作用因子网络调控。母体的基因和表观遗传组成对这一环境的影响尚不清楚。在一个由母亲和16个月大幼儿组成的队列中（n = 65对母婴），我们研究了母体脂肪酸去饱和酶2（FADS2）的基因和表观遗传特征与幼儿n-6和n-3脂肪酸代谢之间的关联。我们检测了母亲和幼儿的FADS2 rs174575变异及DNA甲基化状态，以及幼儿的食物摄入量和血浆脂肪酸浓度。多变量拟合模型表明，母体rs174575基因型与DNA甲基化相结合，能够预测所有幼儿的α-亚麻酸血浆浓度以及男孩的花生四烯酸浓度。花生四烯酸摄入量可预测女孩体内其血浆浓度，而3种主要n-3脂肪酸（二十碳五烯酸、二十二碳五烯酸和二十二碳六烯酸）的摄入量可预测男孩体内它们的血浆浓度。幼儿的FADS2基因型和DNA甲基化与血浆浓度或食物摄入量无关，但CpG8甲基化除外。母体FADS2甲基化可预测男孩的α-亚麻酸摄入量。这项探索性研究表明，母体FADS2的基因和表观遗传状态可能与幼儿的多不饱和脂肪酸代谢有关。
