Rahlouni Fatima, Szarka Szabolcs, Shulaev Vladimir, Prokai Laszlo
1 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center , Fort Worth, Texas.
2 Department of Biological Sciences, University of North Texas , Denton, Texas.
Zebrafish. 2015 Dec;12(6):398-407. doi: 10.1089/zeb.2015.1121. Epub 2015 Oct 6.
Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect of this procedure on the remaining proteome has not been fully studied. Here, we report a label-free shotgun proteomics survey on proteome coverage and biological processes that are enriched and depleted as a result of deyolking. Enriched proteins are involved in cellular energetics and development pathways, specifically implicating enrichment related to mitochondrial function. Although few proteins were removed completely by deyolking, depleted molecular pathways were associated with calcium signaling and signaling events implicating immune system response.
去除斑马鱼（Danio rerio）胚胎中最丰富的蛋白质——脱卵黄，是一种用于深入探索胚胎发育阶段因各种环境应激源或药物影响而导致的体内蛋白质组水平变化的常用技术。然而，该操作对剩余蛋白质组的影响尚未得到充分研究。在此，我们报告了一项无标记鸟枪法蛋白质组学研究，该研究针对脱卵黄后蛋白质组覆盖范围以及富集和减少的生物学过程进行了调查。富集的蛋白质参与细胞能量代谢和发育途径，特别涉及与线粒体功能相关的富集。虽然很少有蛋白质因脱卵黄而被完全去除，但减少的分子途径与钙信号传导以及涉及免疫系统反应的信号事件有关。
