Ceconi I, Ruiz-Moreno M J, DiLorenzo N, DiCostanzo A, Crawford G I
J Anim Sci. 2015 Aug;93(8):4058-69. doi: 10.2527/jas.2014-8299.
Ruminal degradable intake protein (DIP) deficit may result when cattle are fed diets containing a greater inclusion of processed corn grain and small to moderate inclusion of corn distillers grains (DG). This deficit may arise from greater proportions of rapidly fermentable carbohydrates and RUP in corn grain. Urea-derived N is 100% DIP; however, rates of degradation of carbohydrates and conventional urea (CU) may not match. Therefore, beneficial effects may result from the use of slow-release urea (SRU) sources over CU when added to DIP-deficient diets. An experiment was conducted to evaluate the effect of increasing DIP concentration through inclusion of 1 of 2 SRU sources or CU in DG-containing feedlot diets on ruminal fermentation and total tract digestibility. In addition, an in situ experiment was conducted to characterize N disappearance of urea sources from polyester bags. Four ruminally cannulated steers (initial BW = 588 ± 8 kg) were arranged in a 4 × 4 Latin square design and assigned randomly to 1 of 4 dietary treatments containing 0% (CON) or 0.6% urea in the form of CU (UREA) or SRU as Optigen II (polymer-encapsulated urea; OPTI) or NitroShure (lipid-encapsulated urea; NITRO), and 30% corn earlage, 20% modified corn DG with solubles, 7.8% corn silage, 4.3% dry supplement, and dry-rolled corn (DM basis). Dietary DIP was estimated at 6.6% and 8.3% for CON and urea-containing dietary treatments, respectively. Steers were fed ad libitum once daily. Differences in purine derivatives-to-creatinine (PDC) index between treatments were used as indicators of differences in microbial CP synthesis. Intake of OM, digestibility of OM, NDF, CP, and starch, ruminal pH, total VFA ruminal concentration, and PDC index were not affected by treatment ( ≥ 0.21). Concentration of ammonia-N noticeably peaked at 4 h after feed delivery for cattle fed UREA (treatment × time, = 0.06) and measured at least 5.5 mg/dL for any treatment and at any hour after feed delivery. During the first 12 h after incubation, N disappearance was greater for CU and NitroShure than Optigen II (urea source × time, < 0.01). Supplementing DIP through inclusion of CU or SRU did not affect feed intake, digestibility, or most of the ruminal fermentation parameters evaluated, which may relate to the lack of need of urea supplementation in the present experiment. More research is warranted to evaluate the use of SRU in DIP-deficient diets.
当给牛饲喂含有较高比例加工玉米谷物和少量至中等比例玉米酒糟(DG)的日粮时,可能会出现瘤胃可降解摄入蛋白(DIP)不足的情况。这种不足可能源于玉米谷物中快速发酵碳水化合物和RUP的比例较高。尿素衍生的氮是100%的DIP;然而,碳水化合物和常规尿素(CU)的降解速率可能不匹配。因此,在添加到DIP缺乏的日粮中时,使用缓释尿素(SRU)来源而非CU可能会产生有益效果。进行了一项实验,以评估在含DG的育肥牛日粮中添加两种SRU来源之一或CU来提高DIP浓度对瘤胃发酵和全肠道消化率的影响。此外,还进行了一项原位实验,以表征聚酯袋中尿素来源的氮消失情况。将四头装有瘤胃瘘管的阉牛(初始体重 = 588 ± 8千克)按4×4拉丁方设计安排,并随机分配到4种日粮处理中的一种,日粮处理中含有0%(对照)或0.6%以CU(UREA)形式存在的尿素,或以Optigen II(聚合物包被尿素;OPTI)或NitroShure(脂质包被尿素;NITRO)形式存在的SRU,以及30%的玉米青贮、20%的含可溶物的改性玉米DG、7.8%的玉米青贮、4.3%的干补充料和干碾压玉米(以干物质计)。对照和含尿素日粮处理的日粮DIP估计分别为6.6%和8.3%。阉牛每天自由采食一次。处理之间嘌呤衍生物与肌酐(PDC)指数的差异用作微生物CP合成差异的指标。处理对有机物摄入量、有机物消化率、中性洗涤纤维、粗蛋白和淀粉消化率、瘤胃pH值、瘤胃总挥发性脂肪酸浓度和PDC指数均无影响(P≥0.21)。饲喂UREA的牛在采食后4小时氨氮浓度明显达到峰值(处理×时间,P = 0.06),且在采食后任何时间任何处理的氨氮浓度至少为5.5毫克/分升。在孵育后的前12小时内,CU和NitroShure的氮消失量大于Optigen II(尿素来源×时间,P<0.01)。通过添加CU或SRU补充DIP对采食量、消化率或所评估的大多数瘤胃发酵参数均无影响,这可能与本实验中对尿素补充的需求不足有关。有必要进行更多研究来评估SRU在DIP缺乏日粮中的应用。