Gonzalez-Reyes J A, Gracia-Navarro F, Garcia-Herdugo G, Hidalgo A, Navas P
Departamento de Biología Celular, Facultad de Ciencias, Universidad de Córdoba, Spain.
J Electron Microsc Tech. 1989 Jan;11(1):1-8. doi: 10.1002/jemt.1060110102.
In order to determine the best conditions to carry out quantitative ultrastructural studies in plant specimens, five different fixation techniques, including some of the most reported electron microscopy fixatives (glutaraldehyde-paraformaldehyde, osmium tetroxide, potassium permanganate), were assayed in onion root meristems to check their ability to induce morphometric changes in Golgi apparatus ultrastructure. Although the parameters evaluated showed in all cases the same tendency, values obtained after permanganate fixation were always higher than those found after aldehyde techniques (especially aldehyde-osmium). Aldehyde followed by osmium fixation appears as the most indicated fixation method when accurate quantitative ultrastructural studies are to be developed.
为了确定对植物标本进行定量超微结构研究的最佳条件，在洋葱根尖分生组织中测试了五种不同的固定技术，包括一些文献中报道最多的电子显微镜固定剂（戊二醛-多聚甲醛、四氧化锇、高锰酸钾），以检查它们诱导高尔基体超微结构形态计量学变化的能力。尽管所有情况下评估的参数都显示出相同的趋势，但高锰酸钾固定后获得的值总是高于醛类技术（尤其是醛-锇）后获得的值。当要开展精确的定量超微结构研究时，醛类固定后再用锇固定似乎是最适用的固定方法。
