Mann D R, Adams S R, Gould K G, Orr T E, Collins D C
Department of Physiology, Morehouse School of Medicine, Atlanta, GA 30310.
J Reprod Fertil. 1989 Jan;85(1):89-95. doi: 10.1530/jrf.0.0850089.
In Exp. 1, the effect of treatment with a GnRH agonist on basal concentrations of serum testosterone and peak values of serum testosterone after administration of hCG was determined. One group of adult male monkeys was treated with a low dose (5-10 micrograms/day) and a second group with a high dose (25 micrograms/day) of a GnRH agonist for 44 weeks. Basal and peak testosterone concentrations were both significantly reduced by GnRH agonist treatment in all groups compared to untreated control animals, but the % rise in serum testosterone above basal values in response to hCG administration was unchanged by agonist treatment. In Exp. 2, the GnRH agonist (100 or 400 ng) or a GnRH antagonist (4 micrograms) was infused into the testicular arteries of adult monkeys. The agonist did not alter testosterone concentrations in the testicular vein or testosterone and LH values in the femoral vein. In Exp. 3, testicular interstitial cells from monkeys were incubated with three concentrations (10(-9), 10(-7) and 10(-5)M) of the GnRH agonist or a GnRH antagonist with and without hCG. After 24 h, neither basal nor hCG-stimulated testosterone production was affected by the presence of the GnRH agonist or antagonist. The results from all 3 experiments clearly suggest that GnRH agonist treatment does not directly alter steroid production by the monkey testis.
在实验1中，测定了促性腺激素释放激素（GnRH）激动剂治疗对血清睾酮基础浓度以及人绒毛膜促性腺激素（hCG）给药后血清睾酮峰值的影响。一组成年雄性猴子接受低剂量（5 - 10微克/天）的GnRH激动剂治疗，另一组接受高剂量（25微克/天）的GnRH激动剂治疗，为期44周。与未治疗的对照动物相比，所有组中GnRH激动剂治疗均使睾酮的基础浓度和峰值浓度显著降低，但激动剂治疗并未改变hCG给药后血清睾酮相对于基础值的升高百分比。在实验2中，将GnRH激动剂（100或400纳克）或GnRH拮抗剂（4微克）注入成年猴子的睾丸动脉。该激动剂未改变睾丸静脉中的睾酮浓度或股静脉中的睾酮和促黄体生成素（LH）值。在实验3中，将猴子的睾丸间质细胞与三种浓度（10⁻⁹、10⁻⁷和10⁻⁵摩尔/升）的GnRH激动剂或GnRH拮抗剂一起孵育，同时加入或不加入hCG。24小时后，GnRH激动剂或拮抗剂的存在对基础睾酮生成以及hCG刺激的睾酮生成均无影响。所有3个实验的结果均清楚表明，GnRH激动剂治疗不会直接改变猴子睾丸的类固醇生成。
