Medeiros-Neves Bruna, de Barros Francisco Maikon Corrêa, von Poser Gilsane Lino, Teixeira Helder Ferreira
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil.
Molecules. 2015 Oct 2;20(10):18083-94. doi: 10.3390/molecules201018083.
Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584-54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3'-methyl-2',3'-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2',3'-epoxy-3'-methylbutyloxy)coumarin.
翼茎草属植物通常被用作抗真菌和伤口愈合剂。这些活性与香豆素有关，香豆素在这些植物中含量丰富。香豆素可溶于有机溶剂，如己烷和二氯甲烷，其中一些也可溶于热水。鉴于这些植物的浸剂和煎剂在传统医学中被使用，本研究的目的是鉴定和定量巴拉那翼茎草水提取物中的香豆素。水提取物通过动态浸渍法获得，化合物通过超高效液相色谱-紫外-质谱分析进行表征。通过用水提取物与正己烷进行分配，得到了一种新的香豆素和用于验证高效液相色谱分析方法的5-甲氧基-6,7-亚甲二氧基香豆素。经验证的高效液相色谱方法具有线性、特异性和精确性。在水提取物中鉴定出七种香豆素，其含量范围为0.584 - 54毫克/克干植物材料。主要化合物5,6-二甲氧基-7-(3'-甲基-2',3'-二羟基丁氧基)香豆素与一种新化合物5,6-二甲氧基-7-(2',3'-环氧-3'-甲基丁氧基)香豆素首次在巴拉那翼茎草中被描述。
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