Martínez-Rivas Claudia Janeth, Álvarez-Román Rocío, Rivas-Morales Catalina, Elaissari Abdelhamid, Fessi Hatem, Galindo-Rodríguez Sergio Arturo
Facultad de Ciencias Biológicas, Laboratorio de Nanotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba s/n, 66455 San Nicolás de los Garza, NL, Mexico.
Univ Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEP-UMR 5007, 69622 Lyon, France.
J Anal Methods Chem. 2017;2017:9086467. doi: 10.1155/2017/9086467. Epub 2017 Nov 22.
The interest on plants has been focalized due to their biological activities. Extracts or fractions from plants in biodegradable polymeric nanoparticles (NP) provide many advantages on application studies. The encapsulation of the extract or fraction in NP is determined for the establishment of the test dose. HPLC method is an alternative to calculate this parameter. An analytical method based on HPLC for quantification of a hexane fraction from was developed and validated according to ICH. Different concentrations of the hexane fraction from leaves (HFL) were prepared (100-600 g/mL). Linearity, limit of detection, limit of quantification, and intra- and interday precision parameters were determined. HFL was encapsulated by nanoprecipitation technique and analyzed by HPLC for quantitative aspect. The method was linear and precise for the quantification of the HFL components. NP size was 190 nm with homogeneous size distribution. Through validation method, it was determined that the encapsulation of components (1), (2), (3), and (4) was 44, 74, 86, and 97%, respectively. A simple, repeatable, and reproducible methodology was developed for the propose of quantifying the components of a vegetable material loaded in NP, using as a model the hexane fraction of leaves.
由于植物的生物活性，人们对植物的兴趣日益集中。可生物降解聚合物纳米颗粒（NP）中的植物提取物或馏分在应用研究中具有许多优势。将提取物或馏分封装在NP中是为了确定测试剂量。HPLC方法是计算该参数的一种替代方法。根据国际人用药品注册技术协调会（ICH）开发并验证了一种基于HPLC的分析方法，用于定量来自[植物名称未给出]的己烷馏分。制备了不同浓度的叶己烷馏分（HFL）（100 - 600μg/mL）。测定了线性、检测限、定量限以及日内和日间精密度参数。通过纳米沉淀技术封装HFL，并通过HPLC进行定量分析。该方法对于HFL成分的定量具有线性和精确性。NP尺寸为190nm，尺寸分布均匀。通过验证方法确定，成分（1）、（2）、（3）和（4）的封装率分别为44%、74%、86%和97%。以[植物名称未给出]叶的己烷馏分为模型，开发了一种简单、可重复且可重现的方法，用于定量NP中负载的植物材料的成分。
