Ishikawa I, Horiguchi T, Shikura N
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
Nephron. 1989;51(2):211-4. doi: 10.1159/000185287.
The origins of acquired cysts, hyperplastic epithelia of cyst walls, and renal cell carcinomas were investigated by evaluating their lectin conjugate reactivity. Paraffin-embedded blocks from 9 patients with acquired cystic disease were examined by the high-sensitivity lectin-antilectin immune peroxidase method. 11-176 lesions in each patient, 690 lesions in total, were stained both with Tetragonolobus lotus lectin (T) and peanut lectin (P); the former is specific for proximal tubules and the latter for distal tubules and collecting ducts. Out of 606 acquired cysts with single-layered epithelia, 559 (92.2%) were positive for T and negative for P, and 66 out of 75 (88.0%) cysts with hyperplastic multilayered epithelia were positive for T and negative for P. Three out of 4 solid adenomas and, to varying degrees, 5 renal carcinomas revealed the same reaction. These results suggest that almost all cysts accompanying acquired cystic disease of the kidney, including those with single-layered and multilayered epithelia, as well as solid adenomas and renal cell carcinomas, are derived from proximal tubules.
通过评估凝集素结合物反应性，对获得性囊肿、囊肿壁增生上皮及肾细胞癌的起源进行了研究。采用高灵敏度凝集素 - 抗凝集素免疫过氧化物酶法，对9例获得性囊性疾病患者的石蜡包埋组织块进行检查。每位患者有11 - 176个病变，共计690个病变，用四角豆凝集素（T）和花生凝集素（P）进行染色；前者对近端小管具有特异性，后者对远端小管和集合管具有特异性。在606个单层上皮的获得性囊肿中，559个（92.2%）T染色阳性而P染色阴性，75个多层增生上皮囊肿中的66个（88.0%）T染色阳性而P染色阴性。4个实性腺瘤中的3个以及5个肾癌在不同程度上呈现相同反应。这些结果表明，几乎所有伴随肾脏获得性囊性疾病的囊肿，包括单层和多层上皮的囊肿，以及实性腺瘤和肾细胞癌，均起源于近端小管。
