针对牛和羊物种开发的单核苷酸多态性（SNP）芯片在驯鹿（Rangifer tarandus）全基因组分析中的适用性研究。

A Study of Applicability of SNP Chips Developed for Bovine and Ovine Species to Whole-Genome Analysis of Reindeer Rangifer tarandus.

作者信息

Kharzinova Veronika R, Sermyagin Alexander A, Gladyr Elena A, Okhlopkov Innokentiy M, Brem Gottfried, Zinovieva Natalia A

机构信息

From the L.K. Ernst Institute for Animal Husbandry, Dubrovitzy Estate, 60, Podolsk district, Moscow region, Podolsk 142132, Russia (Kharzinova, Sermyagin, Gladyr, Brem, and Zinovieva); Science Institute of Biological Problems Cryolithozone, Yakutsk 677980, Russia (Okhlopkov); and Institute for Animal Breeding and Genetics, VMU, A-1210 Vienna, Austria (Brem).

出版信息

J Hered. 2015 Nov-Dec;106(6):758-61. doi: 10.1093/jhered/esv081. Epub 2015 Oct 7.

DOI:10.1093/jhered/esv081

PMID:26447215

Abstract

Two sets of commercially available single nucleotide polymorphisms (SNPs) developed for cattle (BovineSNP50 BeadChip) and sheep (OvineSNP50 BeadChip) have been trialed for whole-genome analysis of 4 female samples of Rangifer tarandus inhabiting Russia. We found out that 43.0% of bovine and 47.0% of Ovine SNPs could be genotyped, while only 5.3% and 2.03% of them were respectively polymorphic. The scored and the polymorphic SNPs were identified on each bovine and each ovine chromosome, but their distribution was not unique. The maximal value of runs of homozygosity (ROH) was 30.93Mb (for SNPs corresponding to bovine chromosome 8) and 80.32Mb (for SNPs corresponding to ovine chromosome 7). Thus, the SNP chips developed for bovine and ovine species can be used as a powerful tool for genome analysis in reindeer R. tarandus.

摘要

已对两组分别为牛（牛SNP50芯片）和绵羊（羊SNP50芯片）开发的市售单核苷酸多态性（SNP）进行了试验，用于对4份栖息在俄罗斯的驯鹿雌性样本进行全基因组分析。我们发现，43.0%的牛SNP和47.0%的羊SNP能够进行基因分型，而其中分别只有5.3%和2.03%是多态性的。在每条牛染色体和每条羊染色体上都鉴定出了已评分的和多态性的SNP，但它们的分布并非唯一。纯合子片段（ROH）的最大值分别为30.93Mb（对应牛的8号染色体的SNP）和80.32Mb（对应羊的7号染色体的SNP）。因此，为牛和羊物种开发的SNP芯片可作为驯鹿全基因组分析的有力工具。