Grami Raoudha, Mansour Wejdene, Ben Haj Khalifa Anis, Dahmen Safia, Chatre Pierre, Haenni Marisa, Aouni Mahjoub, Madec Jean-Yves
1 Unité Antibiorésistance et Virulence Bactériennes , ANSES Site de Lyon, Lyon, France .
2 Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives , Faculté de Pharmacie, Monastir, Tunisie.
Microb Drug Resist. 2016 Mar;22(2):137-40. doi: 10.1089/mdr.2014.0278. Epub 2015 Oct 8.
Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum β-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited blaCTX-M-15 and blaTEM-1 on an untypeable plasmid and blaOXA-48 on an IncL/M plasmid. KP2 was genetically unrelated to KP1 (ST147) and harbored an IncA/C plasmid carrying blaCMY-4 and the blaOXA-48 derivative gene: blaOXA-204. This study reports the second case worldwide of an OXA-204-producing K. pneumoniae isolate from the same country, however, in a different genetic background.
对来自突尼斯塔哈尔·斯法尔大学医院的首例耐碳青霉烯类肺炎克雷伯菌分离株（KP1和KP2）的分子特征进行了研究。进行了抗菌药物敏感性测试、多位点序列分型、S1核酸酶脉冲场凝胶电泳、Southern印迹分析以及基于聚合酶链反应（PCR）的复制子分型。通过PCR和测序检测了超广谱β-内酰胺酶和碳青霉烯酶基因。两株分离株均对多种药物耐药。KP1为序列型（ST）ST101，在一个不可分型的质粒上携带blaCTX-M-15和blaTEM-1，在一个IncL/M质粒上携带blaOXA-48。KP2与KP1在基因上不相关（ST147），并携带一个携带blaCMY-4和blaOXA-48衍生基因blaOXA-204的IncA/C质粒。本研究报告了全球第二例来自同一国家但遗传背景不同的产OXA-204肺炎克雷伯菌分离株。
