来自巴西棘口吸虫皮层的T1和T2分泌小体的超微结构和细胞化学特征

Ultrastructural and cytochemical characterization of T1 and T2 secretory bodies from the tegument of Echinostoma paraensei.

作者信息

Gonçalves Júlia P, Oliveira-Menezes Aleksandra, Maldonado Junior Arnaldo, Carvalho Técia M U, de Souza Wanderley

机构信息

Pólo Barreto, Universidade Federal do Rio de Janeiro, Campus Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, Rio de Janeiro CEP: 27910-970, Brazil; Laboratório de Biologia de Helmintos Otto Wucherer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil.

Pólo Barreto, Universidade Federal do Rio de Janeiro, Campus Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, Rio de Janeiro CEP: 27910-970, Brazil.

出版信息

Micron. 2016 Jan;80:59-65. doi: 10.1016/j.micron.2015.09.008. Epub 2015 Sep 21.

DOI:10.1016/j.micron.2015.09.008

PMID:26451647

Abstract

Trematodes are lined by a syncytial layer that is named the tegument and contains small mitochondria and two different kinds of secretory inclusions. The structure and size of these bodies differs among genera and species. In a previous study, we observed many secretory bodies in the tegument of Echinostoma paraensei and named these bodies the T1 and T2 secretory bodies. No previous studies analyzed the secretory bodies of trematodes from the genus Echinostoma. Thus, the aim of this work was to use electron microscopy and cytochemistry to characterize these secretory bodies and to provide a detailed ultrastructural and morphological picture of these bodies, which are found in the tegument of E. paraensei. After ultrastructural cytochemistry analysis, we showed that both the T1 and T2 secretory bodies of E. paraensei were formed by glycoconjugates. 3D reconstruction confirmed the ovoid form of T1 secretory bodies and the biconcave and thin form of T2 secretory bodies.

摘要

吸虫体表有一层合胞体层，称为皮层，含有小线粒体和两种不同类型的分泌内含物。这些物体的结构和大小在不同属和种之间有所不同。在之前的一项研究中，我们在副睾棘口吸虫的皮层中观察到许多分泌小体，并将这些小体命名为T1和T2分泌小体。以前没有研究分析过棘口吸虫属吸虫的分泌小体。因此，这项工作的目的是利用电子显微镜和细胞化学来表征这些分泌小体，并提供这些存在于副睾棘口吸虫皮层中的小体的详细超微结构和形态图。经过超微结构细胞化学分析，我们发现副睾棘口吸虫的T1和T2分泌小体均由糖缀合物形成。三维重建证实了T1分泌小体的卵形形态以及T2分泌小体的双凹且薄的形态。