Ren Chunguang, Nagao Satoshi, Yamanaka Masaru, Komori Hirofumi, Shomura Yasuhito, Higuchi Yoshiki, Hirota Shun
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Faculty of Education, Kagawa University, 1-1 Saiwai, Takamatsu, Kagawa 760-8522, Japan.
Mol Biosyst. 2015 Dec;11(12):3218-21. doi: 10.1039/c5mb00545k.
High-order oligomers of Hydrogenobacter thermophilus cytochrome c552 increased with the insertion of more Gly residues between Ala18 and Lys19 at the major hinge loop of the wild-type protein. N-Terminal domain swapping and C-terminal domain swapping were elucidated by using X-ray crystallography for the mutant with the insertion of three Gly residues at the hinge loop.
