Molina-Calle María, Priego-Capote Feliciano, Luque de Castro María D
Department of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, 14071 Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Córdoba (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14071 Córdoba, Spain; University of Córdoba, Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, 14071 Córdoba, Spain.
Department of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, 14071 Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Córdoba (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14071 Córdoba, Spain; University of Córdoba, Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, 14071 Córdoba, Spain.
Talanta. 2015 Nov 1;144:349-55. doi: 10.1016/j.talanta.2015.05.054. Epub 2015 Jun 23.
Peel, a part of the citrus rich in compounds with high-added value, constitutes the bulk of the waste generated in citrus juice industries. Flavonoids are a class of these high-added value compounds characterized by their bioactivity. In this research, a method for analysis of flavonoids, based on LC-MS/MS by using a triple quadrupole detector, has been developed and applied to the quantitative analysis of 16 flavonoids in extracts obtained by maceration of citrus peel. The parameters involved in the ionization and fragmentation of the target analytes were optimized to develop a selected reaction monitoring (SRM) method, which reported detection and quantitation limits ranging from 0.005 to 5 ng/mL and from 0.01 to 10 ng/mL, respectively. The raw materials for flavonoids extraction were fresh, oven-dried and lyophilized peel of 8 different orange varieties, and the proposed quantitation method was applied to the analysis of the obtained extracts. Evaluation of the two methods of water removal showed that lyophilization preserves the concentration of the flavonoids, while oven-dried peel presented a decrease of glycosylated flavonoids and an increase of aglycone forms.
果皮是柑橘中富含高附加值化合物的部分，也是柑橘汁产业产生的主要废弃物。黄酮类化合物是这类具有生物活性的高附加值化合物中的一类。在本研究中，开发了一种基于液相色谱-串联质谱（LC-MS/MS）并使用三重四极杆检测器的黄酮类化合物分析方法，并将其应用于柑橘皮浸渍提取物中16种黄酮类化合物的定量分析。对目标分析物的电离和碎片化所涉及的参数进行了优化，以开发一种选择反应监测（SRM）方法，该方法报告的检测限和定量限分别为0.005至5 ng/mL和0.01至10 ng/mL。黄酮类化合物提取的原料是8个不同橙子品种的新鲜、烘干和冻干果皮，并将所提出的定量方法应用于所得提取物的分析。对两种脱水方法的评估表明，冻干可保留黄酮类化合物的浓度，而烘干的果皮中糖基化黄酮类化合物减少，苷元形式增加。
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