微小RNA命名法：一种融合遗传起源、生物合成途径和序列变体的观点。
miRNA Nomenclature: A View Incorporating Genetic Origins, Biosynthetic Pathways, and Sequence Variants.
作者信息
Desvignes T, Batzel P, Berezikov E, Eilbeck K, Eppig J T, McAndrews M S, Singer A, Postlethwait J H
机构信息
Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA.
European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9713 AV Groningen, The Netherlands.
出版信息
Trends Genet. 2015 Nov;31(11):613-626. doi: 10.1016/j.tig.2015.09.002. Epub 2015 Oct 8.
High-throughput sequencing of miRNAs has revealed the diversity and variability of mature and functional short noncoding RNAs, including their genomic origins, biogenesis pathways, sequence variability, and newly identified products such as miRNA-offset RNAs (moRs). Here we review known cases of alternative mature miRNA-like RNA fragments and propose a revised definition of miRNAs to encompass this diversity. We then review nomenclature guidelines for miRNAs and propose to extend nomenclature conventions to align with those for protein-coding genes established by international consortia. Finally, we suggest a system to encompass the full complexity of sequence variations (i.e., isomiRs) in the analysis of small RNA sequencing experiments.
对微小RNA（miRNA）进行高通量测序，揭示了成熟且具有功能的短非编码RNA的多样性和变异性，包括它们的基因组起源、生物合成途径、序列变异性，以及新发现的产物，如miRNA偏移RNA（moR）。在此，我们回顾了替代性成熟miRNA样RNA片段的已知案例，并提出了一个修订后的miRNA定义，以涵盖这种多样性。然后，我们回顾了miRNA的命名指南，并建议扩展命名惯例，使其与国际联盟建立的蛋白质编码基因的命名惯例保持一致。最后，我们提出了一个系统，以在分析小RNA测序实验时涵盖序列变异（即异源miRNA）的全部复杂性。
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