Gilkeson G S, Grudier J P, Karounos D G, Pisetsky D S
Medical Research Service, Durham Veterans Administration Hospital, North Carolina 27710.
J Immunol. 1989 Mar 1;142(5):1482-6.
Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.
由于近期观察表明细菌DNA具有免疫原性，因此对正常小鼠中抗大肠杆菌（EC）双链DNA抗体的诱导情况进行了研究。将BALB/c和C57BL/6小鼠用与甲基化牛血清白蛋白（BSA）复合的双链EC或双链小牛胸腺（CT）DNA在佐剂中进行免疫；通过酶联免疫吸附测定（ELISA）测量抗体反应。在这两种品系中，双链EC DNA免疫诱导的针对双链EC DNA的抗双链DNA反应比双链CT DNA免疫诱导的反应高得多。当用双链CT DNA进行检测时，两个免疫组均未显示出明显的抗体反应。还通过使用合成DNA双链体的ELISA以及使用3H标记的双链EC DNA作为抗原的滤膜结合试验证明了抗双链DNA抗体。这些结果表明细菌双链DNA具有免疫原性，并且至少一些抗双链DNA特异性可以通过免疫产生。
