间接免疫荧光法检测自身免疫性荨麻疹
Indirect Immunofluorescence for the Detection of Autoimmune Urticaria.
作者信息
Bahrani Bahar, Gattey Natasha T, Hull Peter R
机构信息
Division of Dermatology, Department of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Division of Dermatology, Department of Medicine, University of Saskatchewan, Saskatoon, SK, Canada
出版信息
J Cutan Med Surg. 2016 Mar-Apr;20(2):113-7. doi: 10.1177/1203475415610745. Epub 2015 Oct 9.
BACKGROUND
An autoimmune basis is believed to be responsible for about half of chronic spontaneous urticaria (CSU) cases. The autologous serum skin test is used as a possible indicator, but there is currently no test that directly indicates an autoimmune etiology. In this study, an indirect immunofluorescence was used to identify patients with autoantibodies directed at mast cells.
METHODS
Two substrates were used including paraffin embedded sections of skin biopsies from an infant with bullous mastocytosis and cord blood-derived mast cells (CBMC). Sera from 76 patients with CSU were incubated with substrates and conjugated with human IgG.
RESULTS
Using the bullous mastocytosis preparations, positive indirect immunofluorescence was found in 46% (n = 76), while the CBMC substrate was positive in 39% (n = 70).
CONCLUSION
The IgG autoantibodies directed at mast cells could be detected in about half the patients with CSU. Indirect immunofluorescence should be considered as an indicator of the autoimmune form of CSU.
背景
自身免疫基础被认为是约一半慢性自发性荨麻疹（CSU）病例的病因。自体血清皮肤试验被用作一种可能的指标，但目前尚无直接表明自身免疫病因的检测方法。在本研究中，采用间接免疫荧光法来识别针对肥大细胞的自身抗体患者。
方法
使用了两种底物，包括来自一名大疱性肥大细胞增多症婴儿的皮肤活检石蜡包埋切片和脐带血来源的肥大细胞（CBMC）。将76例CSU患者的血清与底物孵育，并与人IgG结合。
结果
使用大疱性肥大细胞增多症制剂时，46%（n = 76）的患者间接免疫荧光呈阳性，而CBMC底物在39%（n = 70）的患者中呈阳性。
结论
约一半的CSU患者可检测到针对肥大细胞的IgG自身抗体。间接免疫荧光应被视为CSU自身免疫形式的一个指标。
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