Steijlen P M, Hamm H, van Erp E J, Johnson J P, Ruiter D J, Bröcker E B
Department of Dermatology, University of Münster, Federal Republic of Germany.
J Invest Dermatol. 1989 Mar;92(3):366-70. doi: 10.1111/1523-1747.ep12277206.
Monoclonal antibodies (MoAbs) against six well-defined progression-associated melanoma antigens (PAMAs) and a MoAb against the nuclear proliferation-associated antigen Ki67 were used for an immunoperoxidase study on 44 biopsies from 36 congenital melanocytic nevi (CMN). Twenty-nine common acquired nevi and 16 metastasizing primary melanomas were studied as controls. Two CMN of the series were giant CMN (greater than 20 cm), one of which later progressed to metastasizing melanoma. Of the remaining 34 CMN, four were histologically associated with a malignant melanoma. DNA flow cytometry was performed on adjacent cryostat sections of 37 biopsies from 28 CMN without melanoma. PAMAs were found expressed in CMN in the following frequencies: A-1-43: 36%; P358: 27%; PAL-M1: 20%; HLA-DR: 9%; Muc 18: 2%; A-10-33: 0%. No PAMAs were present in only 15 biopsies (34%). One single PAMA was found in 20 lesions (45%), and two or more PAMAs in nine lesions (21%). In all four CMN histologically associated with melanoma, and also in one biopsy from the giant CMN which later progressed to melanoma, at least two PAMAs were expressed. The latter biopsy was the only CMN in which the proliferation-associated antigen could be demonstrated. Only this biopsy and a single biopsy of the other giant CMN showed aneuploidy. We found expression of single PAMAs and co-expression of PAMAs in CMN in higher frequency than in common acquired melanocytic nevi. Co-expression of PAMAs was a feature of metastasizing primary melanomas. We conclude that co-expression of two or more PAMAs in a CMN might indicate its malignant potential, because this was found in all CMN proven to have progressed to malignant melanoma.
使用针对六种明确的进展相关黑色素瘤抗原（PAMA）的单克隆抗体（MoAb）和一种针对核增殖相关抗原Ki67的MoAb，对来自36例先天性黑素细胞痣（CMN）的44份活检样本进行免疫过氧化物酶研究。将29例普通获得性痣和16例转移性原发性黑色素瘤作为对照进行研究。该系列中有2例CMN为巨大CMN（大于20 cm），其中1例后来进展为转移性黑色素瘤。在其余34例CMN中，有4例在组织学上与恶性黑色素瘤相关。对来自28例无黑色素瘤的CMN的37份活检样本的相邻低温切片进行了DNA流式细胞术检测。发现PAMA在CMN中的表达频率如下：A-1-43：36%；P358：27%；PAL-M1：20%；HLA-DR：9%；Muc 18：2%；A-10-33：0%。仅15份活检样本（34%）中未检测到PAMA。在20个病变（45%）中发现单一PAMA，在9个病变（21%）中发现两种或更多PAMA。在所有4例组织学上与黑色素瘤相关的CMN以及1例后来进展为黑色素瘤的巨大CMN的活检样本中，至少表达了两种PAMA。后一份活检样本是唯一能检测到增殖相关抗原的CMN。只有这份活检样本和另一例巨大CMN的一份活检样本显示非整倍体。我们发现CMN中单一PAMA的表达和PAMA的共表达频率高于普通获得性黑素细胞痣。PAMA的共表达是转移性原发性黑色素瘤的一个特征。我们得出结论，CMN中两种或更多PAMA的共表达可能表明其恶性潜能，因为在所有已证实进展为恶性黑色素瘤的CMN中均发现了这种情况。
