荧光标记可可靠地识别活的人子宫内膜细胞中的沙眼衣原体，并能快速准确地定量衣原体包涵体形成单位。
Fluorescent labeling reliably identifies Chlamydia trachomatis in living human endometrial cells and rapidly and accurately quantifies chlamydial inclusion forming units.
作者信息
Vicetti Miguel Rodolfo D, Henschel Kevin J, Dueñas Lopez Fiorela C, Quispe Calla Nirk E, Cherpes Thomas L
机构信息
Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
出版信息
J Microbiol Methods. 2015 Dec;119:79-82. doi: 10.1016/j.mimet.2015.10.003. Epub 2015 Oct 23.
Abstract
Chlamydia replication requires host lipid acquisition, allowing flow cytometry to identify Chlamydia-infected cells that accumulated fluorescent Golgi-specific lipid. Herein, we describe modifications to currently available methods that allow precise differentiation between uninfected and Chlamydia trachomatis-infected human endometrial cells and rapidly and accurately quantify chlamydial inclusion forming units.
摘要
衣原体复制需要获取宿主脂质，这使得流式细胞术能够识别积累了荧光高尔基体特异性脂质的衣原体感染细胞。在此，我们描述了对现有方法的改进，这些改进能够精确区分未感染和沙眼衣原体感染的人子宫内膜细胞，并快速准确地定量衣原体包涵体形成单位。
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本文引用的文献
1
NIH Image to ImageJ: 25 years of image analysis.
Nat Methods. 2012 Jul;9(7):671-5. doi: 10.1038/nmeth.2089.
2
CPAF: a Chlamydial protease in search of an authentic substrate.
PLoS Pathog. 2012;8(8):e1002842. doi: 10.1371/journal.ppat.1002842. Epub 2012 Aug 2.
3
Chlamydia trachomatis infection control programs: lessons learned and implications for vaccine development.
Infect Dis Obstet Gynecol. 2011;2011:754060. doi: 10.1155/2011/754060. Epub 2011 Nov 14.
4
Endometrial leukocyte subpopulations associated with Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis genital tract infection.
Am J Obstet Gynecol. 2011 Oct;205(4):324.e1-7. doi: 10.1016/j.ajog.2011.05.031. Epub 2011 May 20.
5
Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.
Nature. 2009 Feb 5;457(7230):731-5. doi: 10.1038/nature07578. Epub 2008 Dec 7.
6
Cultivation and Laboratory Maintenance of Chlamydia trachomatis.
Curr Protoc Microbiol. 2005 Jul;Chapter 11:Unit 11A.1. doi: 10.1002/9780471729259.mc11a01s00.
7
Clonal isolation of chlamydia-infected cells using flow cytometry.
J Microbiol Methods. 2007 Jan;68(1):201-8. doi: 10.1016/j.mimet.2006.07.012. Epub 2006 Sep 25.
8
Sphingomyelin trafficking in Chlamydia pneumoniae-infected cells.
Cell Microbiol. 2001 Mar;3(3):145-52. doi: 10.1046/j.1462-5822.2001.00098.x.
9
Fluorescent labelling of intracellular bacteria in living host cells.
J Microbiol Methods. 2000 May;40(3):265-74. doi: 10.1016/s0167-7012(00)00132-9.
10
Lipid metabolism in Chlamydia trachomatis-infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion.
Proc Natl Acad Sci U S A. 1995 May 23;92(11):4877-81. doi: 10.1073/pnas.92.11.4877.
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