雌激素反应性110K和74K大鼠子宫分泌蛋白在结构上与补体成分C3相关。

The estrogen-responsive 110K and 74K rat uterine secretory proteins are structurally related to complement component C3.

作者信息

Kuivanen P C, Capulong R B, Harkins R N, DeSombre E R

机构信息

Ben May Institute, University of Chicago, IL 60637.

出版信息

Biochem Biophys Res Commun. 1989 Feb 15;158(3):898-905. doi: 10.1016/0006-291x(89)92807-6.

DOI:10.1016/0006-291x(89)92807-6

PMID:2645873

Abstract

Estrogens stimulate the synthesis of specific secretory proteins in the rat uterus. Here we show that two of these, polypeptides of relative molecular weight 110,000 (110K) and 74,000 (74K), are structurally related to C3, the third component of complement, a glycoprotein that plays a central role in regulating complement-mediated inflammatory and immune responses. The similarities were based on the observations that (1) NH2-terminal amino acid sequence of the 74K polypeptide showed sequence homology with the beta chain of mouse C3, (2) comparison of the electrophoretic mobilities of the 110K and 74K polypeptides in the presence and absence of reducing agents revealed that they were disulfide-linked subunits of a protein of Mr approximately 180,000, (3) the native protein was immunoreactive with antibodies specific for rat C3, and (4) both polypeptides were immunoprecipitated with antibodies to rat C3.

摘要

雌激素可刺激大鼠子宫中特定分泌蛋白的合成。在此我们表明，其中两种蛋白，即相对分子质量为110,000（110K）和74,000（74K）的多肽，在结构上与补体的第三成分C3相关，C3是一种糖蛋白，在调节补体介导的炎症和免疫反应中起核心作用。这些相似性基于以下观察结果：（1）74K多肽的氨基末端氨基酸序列与小鼠C3的β链显示出序列同源性；（2）在存在和不存在还原剂的情况下对110K和74K多肽的电泳迁移率进行比较，结果表明它们是Mr约为180,000的蛋白质的二硫键连接亚基；（3）天然蛋白与大鼠C3特异性抗体发生免疫反应；（4）两种多肽均被大鼠C3抗体免疫沉淀。