羊口疮病毒（ORFV）重组体的产生与筛选

Generation and Selection of Orf Virus (ORFV) Recombinants.

作者信息

Rziha Hanns-Joachim, Rohde Jörg, Amann Ralf

机构信息

Institute of Immunology, Friedrich-Loeffler-Institute, Südufer 10, Island of Riems, Greifswald, Germany.

Department of Immunology, Interfaculty Institute of Cell Biology, Eberhard Karls Universität, Auf der Morgenstelle 15, Tübingen, 72076, Germany.

出版信息

Methods Mol Biol. 2016;1349:177-200. doi: 10.1007/978-1-4939-3008-1_12.

DOI:10.1007/978-1-4939-3008-1_12

PMID:26458837

Abstract

Orf virus (ORFV) is an epitheliotropic poxvirus, which belongs to the genus Parapoxvirus. Among them the highly attenuated, apathogenic strain D1701-V is regarded as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, propagation, and titration of ORFV recombinants as well as transgene detection by PCR or immunohistochemical staining.

摘要

羊痘病毒（ORFV）是一种嗜上皮性痘病毒，属于副痘病毒属。其中高度减毒、无致病性的D1701-V株被认为是新型病毒载体疫苗的有前景的候选者。我们最近的工作表明，那些基于ORFV的重组体能够在对ORFV不敏感的各种宿主中诱导保护性的、持久的免疫。在本章中，我们描述了ORFV重组体的产生、筛选、增殖和滴定的程序，以及通过PCR或免疫组织化学染色进行转基因检测的方法。