Rziha H J, Henkel M, Cottone R, Meyer M, Dehio C, Büttner M
Federal Research Centre for Virus Diseases of Animals, Institute for Vaccines, Tübingen, Germany.
J Biotechnol. 1999 Aug 20;73(2-3):235-42. doi: 10.1016/s0168-1656(99)00141-8.
Parapoxvirus (PPV) represents a genus of the poxviridae, and particularly PPV ovis (Orf virus, OV) seems to offer several potential advantages for the use of vector vaccine. Therefore, we started to investigate the genome of the highly attenuated OV strain D1701, which was only poorly characterised until now. Due to recombination of non-homologous sequences, part of the right hand end of the D1701 genome was duplicated and translocated to the opposite end of the genome. As a consequence gene deletion had occurred and the inverted terminal repeat region is increased. Results are described to identify viral genes, which are non-essential for virus replication and potentially influence viral pathogenesis, virulence, and host immunity. In more detail, we analysed the expression and functional activity of the OV-specific vascular endothelial growth factor (VEGF) gene homologue. Finally the construction and production of a D1701 mutant lacking the VEGF gene homologue is reported.
副痘病毒（PPV）是痘病毒科的一个属，特别是羊副痘病毒（羊口疮病毒，OV）似乎在用作载体疫苗方面具有几个潜在优势。因此，我们开始研究高度减毒的OV毒株D1701的基因组，该毒株迄今特征尚不明确。由于非同源序列的重组，D1701基因组右手端的一部分发生了重复并转移到基因组的另一端。结果发生了基因缺失，反向末端重复区域增加。本文描述了鉴定对病毒复制非必需且可能影响病毒发病机制、毒力和宿主免疫的病毒基因的结果。更详细地说，我们分析了OV特异性血管内皮生长因子（VEGF）基因同源物的表达和功能活性。最后报道了缺失VEGF基因同源物的D1701突变体的构建和生产。
