Yen Tzung-Hai, Chen Kuan-Hung, Hsu Min-Yen, Fan Shu-Ting, Huang Yu-Fen, Chang Chia-Ling, Wang Yu-Ping, Cheng Chao-Min
Department of Nephrology and Division of Clinical Toxicology, Chang Gung Memorial Hospital, Linkou Medical Center, Chang Gung University and School of Medicine, Taipei, Taiwan.
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan.
Talanta. 2015 Dec 1;145:66-72. doi: 10.1016/j.talanta.2015.09.041.
This manuscript describes the development and clinical testing of a paper-based, metabolic assay designed for rapid, semi-quantitative measurement of organophosphate poisoning. Paper-based platforms, including point-of-care devices and 96-well plates, provided semi-quantitative information regarding the concentration of AchE (a biomarker for organophosphate poisoning). The paper-based 96-well-plate developed and implemented in this study was used to measure the level of organophosphate poisoning in three different clinical patients. Results were comparable to those obtained using conventional hospital methods currently considered the "gold standard". This diagnostic device offers several advantages over conventional methods, including short operating time (twice as fast as conventional methods), procedure simplicity, and reduced fabrication cost. With further commercialization efforts, the methods described in this manuscript could be applied to a wide range of potential diagnostic applications in the field.
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