Santos Mariane Gonçalves, Moraes Gabriel de Oliveira Isac, Nakamura Maurício Gustavo, dos Santos-Neto Álvaro José, Figueiredo Eduardo Costa
Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000, Alfenas, MG, Brazil.
Laboratory of Chromatography, Institute of Chemistry of São Carlos, University of São Paulo, P.O. Box 780, 13566-590, São Carlos, SP, Brazil.
Analyst. 2015 Nov 21;140(22):7768-75. doi: 10.1039/c5an01482d.
Molecularly imprinting polymers (MIPs) can be modified with external layers in order to obtain restricted access molecularly imprinted polymers (RAMIPs) able to exclude macromolecules and retain low weight compounds. These modifications have been frequently achieved using hydrophilic monomers, chemically bound on the MIP surface. Recently, our group proposed a new biocompatible RAMIP based on the formation of a bovine serum albumin coating on the surface of MIP particles. This material has been used to extract drugs directly from untreated human plasma samples, but its physicochemical evaluation has not been carried out yet, mainly in comparison with RAMIPs obtained by hydrophilic monomers. Thus, we proposed in this paper a comparative study involving the surface composition, microscopic aspect, selectivity, binding kinetics, adsorption and macromolecule elimination ability of these different materials. We concluded that the synthesis procedure influences the size and shape of particles and that hydrophilic co-monomer addition as well as coating with BSA do not alter the chemical recognition ability of the material. The difference between imprinted and non-imprinted polymers' adsorption was evident (suggesting that imprinted polymers have a better capacity to bind the template than the non-imprinted ones). The Langmuir model presents the best fit to describe the materials' adsorption profile. The polymer covered with hydrophilic monomers presented the best adsorption for the template in an aqueous medium, probably due to a hydrophilic layer on its surface. We also concluded that an association of the hydrophilic monomers with the bovine serum albumin coating is important to obtain materials with higher capacity of macromolecule exclusion.
分子印迹聚合物（MIPs）可以用外层进行修饰，以获得能够排除大分子并保留低分子量化合物的受限 access 分子印迹聚合物（RAMIPs）。这些修饰通常使用化学结合在 MIP 表面的亲水性单体来实现。最近，我们小组提出了一种基于在 MIP 颗粒表面形成牛血清白蛋白涂层的新型生物相容性 RAMIP。这种材料已被用于直接从未经处理的人血浆样本中提取药物，但其物理化学评估尚未进行，主要是与通过亲水性单体获得的 RAMIPs 进行比较。因此，我们在本文中提出了一项比较研究，涉及这些不同材料的表面组成、微观外观、选择性、结合动力学、吸附和大分子排除能力。我们得出结论，合成过程会影响颗粒的大小和形状，添加亲水性共聚单体以及用牛血清白蛋白涂层不会改变材料的化学识别能力。印迹聚合物和非印迹聚合物吸附之间的差异很明显（表明印迹聚合物比非印迹聚合物具有更好的结合模板的能力）。朗缪尔模型最适合描述材料的吸附曲线。覆盖有亲水性单体的聚合物在水性介质中对模板的吸附最佳，这可能是由于其表面的亲水性层。我们还得出结论，亲水性单体与牛血清白蛋白涂层的结合对于获得具有更高大分子排除能力的材料很重要。
