日本犬类冠状病毒（CCoV）在犬中的流行情况：CCoV RNA 的检测及回顾性血清学分析
Prevalence of canine coronavirus (CCoV) in dog in Japan: detection of CCoV RNA and retrospective serological analysis.
作者信息
Takano Tomomi, Yamashita Saya, Murata-Ohkubo Michiko, Satoh Kumi, Doki Tomoyoshi, Hohdatsu Tsutomu
机构信息
Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
出版信息
J Vet Med Sci. 2016 Feb;78(2):341-5. doi: 10.1292/jvms.15-0347. Epub 2015 Oct 11.
We collected rectal swabs from dogs in Japan during 2011 to 2014, and canine coronavirus (CCoV) nucleocapsid gene was detected by RT-PCR. The relationship between CCoV infection and the manifestation of diarrhea symptoms was investigated, and a correlation was noted (df=1, χ(2)=8.90, P<0.005). The types of CCoV detected in samples from CCoV-infected dogs were CCoV-I in 88.9% and CCoV-II in 7.4%, respectively. We retrospectively investigated the seroprevalence of CCoV-I in dogs in Japan during 1998 to 2006. The sera were tested with a neutralizing antibody test. In the absence of CCoV-I laboratory strain, we used feline coronavirus (FCoV)-I that shares high sequence homology in the S protein with CCoV-I. 77.7% of the sera were positive for neutralizing anti-FCoV-I antibodies.
2011年至2014年期间，我们从日本的犬只采集了直肠拭子，并通过逆转录聚合酶链反应（RT-PCR）检测犬冠状病毒（CCoV）核衣壳基因。研究了CCoV感染与腹泻症状表现之间的关系，并发现了相关性（自由度=1，χ²=8.90，P<0.005）。在CCoV感染犬只的样本中检测到的CCoV类型，CCoV-I占88.9%，CCoV-II占7.4%。我们回顾性调查了1998年至2006年期间日本犬只中CCoV-I的血清阳性率。血清通过中和抗体试验进行检测。由于缺乏CCoV-I实验室毒株，我们使用了在S蛋白中与CCoV-I具有高度序列同源性的猫冠状病毒（FCoV）-I。77.7%的血清对中和抗FCoV-I抗体呈阳性。
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