Ntafis Vasileios, Mari Viviana, Danika Stefania, Fragkiadaki Eftychia, Buonavoglia Canio
Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece.
J Vet Diagn Invest. 2010 Mar;22(2):320-3. doi: 10.1177/104063871002200231.
Canine coronavirus (CCoV) is usually the cause of mild gastroenteritis in dogs and is known to have spread worldwide. However, to date, no CCoV cases have been confirmed in Greece. In the present work, the authors investigated an outbreak of enteritis in puppies from a Greek kennel for the presence of CCoV. Dogs were presented with clinical signs of diarrhea, anorexia, weakness, depression, dehydration, and 1 death. Canine coronavirus type II was detected by reverse transcription nested polymerase chain reaction in all 11 puppies, whereas 1 puppy presented dual infection with CCoV type II and canine parvovirus 2. Surprisingly, sequence analysis of the samples revealed higher similarity to the pantropic CCoV II strain CB/05 than to other reference strains, in the most variable region of the S gene.
犬冠状病毒（CCoV）通常是犬类轻度肠胃炎的病因，并且已知已在全球范围内传播。然而，迄今为止，希腊尚未确诊CCoV病例。在本研究中，作者调查了希腊一家犬舍中幼犬的肠炎疫情，以检测是否存在CCoV。犬只出现腹泻、厌食、虚弱、抑郁、脱水等临床症状，并有1只死亡。通过逆转录巢式聚合酶链反应在所有11只幼犬中检测到II型犬冠状病毒，而1只幼犬呈现II型CCoV与犬细小病毒2的双重感染。令人惊讶的是，样本的序列分析显示，在S基因最可变区域，与泛嗜性CCoV II毒株CB/05的相似性高于其他参考毒株。
