Carpenter A B, Bartkowiak C D
Division of Clinical Immunopathology, University of Pittsburgh School of Medicine 15213-2583.
Clin Chem. 1989 Mar;35(3):464-6.
Fluorescence immunoassay (FIA), a relatively new technique for measuring rheumatoid factors (RF), is automated, quantitative, and calibrated against the Centers for Disease Control reference material for RF. We studied the FIA method in relation to a panel of RF methods, both qualitative [latex (LA) and sheep cell agglutination (SSCA)], and quantitative [nephelometry and enzyme-linked immunoassay (ELISA)]. Regression analysis revealed a highly significant correlation between FIA and either LA (r = 0.90) or nephelometry (r = 0.87). The correlation between FIA and either SSCA (r = 0.62) or ELISA (r = 0.67) was less strong. FIA had the highest sensitivity (91%) of all these methods; the specificity was 86%. FIA provides an accurate, sensitive, and specific measure of RF, and is a good alternative for laboratories wanting to replace titer methods with automated laboratory analysis.
荧光免疫测定法（FIA）是一种用于测量类风湿因子（RF）的相对较新的技术，它是自动化的、定量的，并以美国疾病控制中心的RF参考物质进行校准。我们研究了FIA方法与一组RF检测方法的关系，包括定性方法[乳胶凝集试验（LA）和绵羊细胞凝集试验（SSCA）]以及定量方法[散射比浊法和酶联免疫吸附测定法（ELISA）]。回归分析显示FIA与LA（r = 0.90）或散射比浊法（r = 0.87）之间存在高度显著的相关性。FIA与SSCA（r = 0.62）或ELISA（r = 0.67）之间的相关性则较弱。FIA在所有这些方法中具有最高的灵敏度（91%）；特异性为86%。FIA提供了一种准确、灵敏且特异的RF检测方法，对于希望用自动化实验室分析取代滴度法的实验室来说是一个很好的选择。
