Zhao Chao, Liu Yali, Li Yuanfang
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University.
Anal Sci. 2015;31(10):1035-9. doi: 10.2116/analsci.31.1035.
Metal-organic framework Fe-MIL-88NH2 as a dual colorimetric and fluorometric sensor has been designed for a wide range of dopamine quantitative detection. It is easy to implement the assay for visual detection of dopamine based on restraining the color change of the 3,3',5,5'-tetramethylbenzidine-H2O2 system that is catalyzed by Fe-MIL-88NH2 with intrinsic peroxidase-like catalytic activity. The linear range is from 50 nM to 30 μM. In addition, the Fe-MIL-88NH2 can exhibit a dramatic decrease of its fluorescent intensity when exposed to dopamine, which may be attributed to the electron transfer from the Fe-MIL-88NH2 to the oxidized dopamine-quinone. The linear response range is from 30 μM to 4 mM. Meanwhile, both colorimetric and fluorometric methods exhibit higher selectivity for DA over a number of possible interfering substances. Furthermore, the proposed method has been successfully applied to detect DA in human serum samples, which suggests its great potential for analytical applications.
金属有机框架Fe-MIL-88NH2作为一种双比色和荧光传感器，已被设计用于广泛的多巴胺定量检测。基于抑制由具有内在过氧化物酶样催化活性的Fe-MIL-88NH2催化的3,3',5,5'-四甲基联苯胺-H2O2体系的颜色变化，很容易实现多巴胺的可视化检测测定。线性范围为50 nM至30 μM。此外，Fe-MIL-88NH2在暴露于多巴胺时其荧光强度会显著降低，这可能归因于电子从Fe-MIL-88NH2转移到氧化的多巴胺-醌。线性响应范围为30 μM至4 mM。同时，比色法和荧光法对多巴胺的选择性均高于多种可能的干扰物质。此外，所提出的方法已成功应用于检测人血清样品中的多巴胺，这表明其在分析应用方面具有巨大潜力。
