下调受体相互作用蛋白激酶1（RIP1）可促进奥沙利铂诱导的Tca8113细胞凋亡。

Down-Regulating Receptor Interacting Protein Kinase 1 (RIP1) Promotes Oxaliplatin-Induced Tca8113 Cell Apoptosis.

作者信息

Shan BaoZhong, Ma Feng, Wang MingGuo, Xu Xin

机构信息

School of Stomatology, Shandong University, Jinan, Shandong, China (mainland).

Department of Stomatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, China (mainland).

出版信息

Med Sci Monit. 2015 Oct 13;21:3089-94. doi: 10.12659/msm.894184.

DOI:10.12659/msm.894184

PMID:26460489

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4610685/

Abstract

BACKGROUND

Oxaliplatin is a crucial chemotherapy drug that plays an important role in colorectal cancer and oral cancer treatment. However, the molecular mechanism of oxaliplatin in killing tongue squamous cell cancer cells is still unknown. This paper investigates the mechanism of by which oxaliplatin regulates tongue squamous cell carcinoma Tca8113 cell survival and death.

MATERIAL AND METHODS

Tca8113 was treated with 1 μmol/L oxaliplatin for 24 h. Tca8113 cell proliferation and apoptosis were determined by MTT method and flow cytometry, respectively. Western blot was applied to detect receptor-interacting protein kinase 1 (RIP1) level. Tca8113 was transfected with siRNA RIP1 and then treated with 1 μmol/L oxaliplatin, and the cell apoptosis was detected.

RESULTS

We found that 1 μmol/L oxaliplatin could inhibit Tca8113 cell growth (cell survival rate was 19.3%), reduce mitochondrial membrane potential (reduce 82.3%) and phosphatidylserine eversion (positive rate was 62.7%), and activate caspase-3 (increased 2.6 times). We also found that 1 μmol/L oxaliplatin treatment could increase RIP1 expression in Tca8113 cells. Cell apoptosis rate increased after siRNA RIP1 and 1 μmol/L oxaliplatin treatment (apoptosis rate was 90.2%).

CONCLUSIONS

Down-regulating RIP1 promotes oxaliplatin induced Tca8113 cells apoptosis.

摘要

背景

奥沙利铂是一种关键的化疗药物，在结直肠癌和口腔癌治疗中发挥着重要作用。然而，奥沙利铂杀伤舌鳞状细胞癌细胞的分子机制仍不清楚。本文研究奥沙利铂调节舌鳞状细胞癌Tca8113细胞存活和死亡的机制。

材料与方法

用1μmol/L奥沙利铂处理Tca8113细胞24小时。分别采用MTT法和流式细胞术检测Tca8113细胞的增殖和凋亡情况。应用蛋白质免疫印迹法检测受体相互作用蛋白激酶1（RIP1）水平。用siRNA RIP1转染Tca8113细胞，然后用1μmol/L奥沙利铂处理，检测细胞凋亡情况。

结果

我们发现1μmol/L奥沙利铂可抑制Tca8113细胞生长（细胞存活率为19.3%），降低线粒体膜电位（降低82.3%）和磷脂酰丝氨酸外翻（阳性率为62.7%），并激活半胱天冬酶-3（增加2.6倍）。我们还发现1μmol/L奥沙利铂处理可增加Tca8113细胞中RIP1的表达。siRNA RIP1和1μmol/L奥沙利铂处理后细胞凋亡率增加（凋亡率为90.2%）。