Brasero Nicolas, Martinet Baptiste, Urbanová Klára, Valterová Irena, Torres Alexandra, Hoffmann Wolfgang, Rasmont Pierre, Lecocq Thomas
University of Mons, Research Institute of Biosciences, Laboratory of Zoology, Place du Parc 20, BE-7000 Mons (phone: +32-65373435).
Academy of Sciences of the Czech Republic, Institute of Organic Chemistry and Biochemistry, Flemingovo nám 2, CZ-166 10 Prague.
Chem Biodivers. 2015 Oct;12(10):1535-46. doi: 10.1002/cbdv.201400375.
The evolution of signals and reproductive traits involved in the pre-mating recognition has been in focus of abundant research in several model species, such as bumblebees (genus Bombus). However, the most-studied bumblebee reproductive trait, the male cephalic labial gland secretions (CLGS), remains unknown among bumblebee species from South America. In this study, the CLGS of five South American bumblebees of the subgenera Thoracobombus (Bombus excellens and B. atratus) and Cullumanobombus (B. rubicundus, B. hortulanus, and B. melaleucus) were investigated, by comparing the chemical compositions of their secretions to those of closely related European species. The results showed an obvious interspecific differentiation in both subgenera. The interspecific differentiation among the species of the Thoracobombus subgenus involved different compounds present at high contents (main compounds), while those of the Cullumanobombus subgenus shared the same main components. This suggests that among the species of the Cullumanobombus subgenus, the differentiation in minor components could lead to species discrimination.
交配前识别所涉及的信号和生殖性状的进化一直是几种模式物种（如熊蜂属（Bombus））大量研究的重点。然而，在南美熊蜂物种中，研究最多的熊蜂生殖性状——雄性头部唇腺分泌物（CLGS）仍然未知。在本研究中，通过比较五种南美熊蜂（胸熊蜂亚属（Thoracobombus）的卓越熊蜂（Bombus excellens）和黑熊蜂（B. atratus）以及库氏熊蜂亚属（Cullumanobombus）的红润熊蜂（B. rubicundus）、园圃熊蜂（B. hortulanus）和白千层熊蜂（B. melaleucus））的CLGS分泌物与亲缘关系相近的欧洲物种的化学成分，对其进行了研究。结果表明，两个亚属中均存在明显的种间分化。胸熊蜂亚属物种间的分化涉及含量较高的不同化合物（主要化合物），而库氏熊蜂亚属物种的主要成分相同。这表明，在库氏熊蜂亚属物种中，次要成分的差异可能导致物种识别。
