Sentman C L, Kumar V, Koo G, Bennett M
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1989 Mar 15;142(6):1847-53.
The rejection of Hh-1 incompatible bone marrow cells in irradiated mice is mediated by NK cells and is genetically regulated. We tested the role of the NK-specific gene, NK1.1, in regulating the rejection of allogeneic bone marrow cell grafts. NK1.1+ mice, that are known to display strong resistance against Hh-1 incompatible grafts, were crossed to H-2/Hh-1 identical NK1.1-, poor responder mice, and the progeny were backcrossed to the poor responder parent. The segregating mice were individually typed for their expression of NK1.1 and the ability to resist Hh-1 incompatible bone marrow cells (BMC). A strong correlation was noted between expression of NK1.1 and rejection of H-2d/Hh-1d BMC. Our results support the idea that NK1.1 is one of the genes responsible for strong resistance to Hh-1d (determinant 2) but not for Hh-1j (determinant 3) BMC grafts. We suggest that the NK1.1 molecule functions as an accessory molecule in the cellular interactions involving the recognition of Hh-1 determinants.
受辐照小鼠对Hh-1不相容骨髓细胞的排斥反应由自然杀伤细胞介导，且受基因调控。我们测试了自然杀伤细胞特异性基因NK1.1在调节同种异体骨髓细胞移植排斥反应中的作用。已知对Hh-1不相容移植物表现出强烈抗性的NK1.1+小鼠与H-2/Hh-1相同的NK1.1-低反应性小鼠杂交，子代再与低反应性亲代回交。对分离的小鼠个体进行NK1.1表达情况以及抵抗Hh-1不相容骨髓细胞（BMC）能力的分型。发现NK1.1的表达与对H-2d/Hh-1d BMC的排斥之间存在强烈相关性。我们的结果支持以下观点：NK1.1是对Hh-1d（决定簇2）而非Hh-1j（决定簇3）BMC移植物产生强烈抗性的基因之一。我们认为NK1.1分子在涉及Hh-1决定簇识别的细胞相互作用中作为辅助分子发挥作用。
